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Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
Comprehensive analysis to identify the relationship between CALD1 and immune infiltration in glioma
Jing Xia1, Qiuan Yang1, Chengwei Wang2
1Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, China.
Background:
An accumulating number of studies show that CALD1 is associated with a variety of tumor microenvironments (TME) and is closely related to patients' survival. However, to the best of our knowledge, few studies examined the role of CALD1 in the immune microenvironment of glioma. The aim of this study is to investigate the potential correlation between CALD1 and the pathogenesis and progression of glioma, aiming to identify a novel therapeutic target.
Methods:
We assessed the role of CALD1 in pan-cancer and investigated the correlation between CALD1 and TME of glioma by bioinformatic analysis and experimental verification.
Results:
We found that CALD1 expression in glioma was associated with a variety of infiltrating immune cells. CALD1 can promote the development of glioma by affecting M2 macrophage infiltration. Also, we found that CALD1 was closely associated with tumor mutation burden, microsatellite instability, copy number variation, methylation, and stem cell index. Our clinical correlation study demonstrated that CALD1 was associated with overall survival, progression-free interval, and disease-specific survival in a variety of tumors. We verified the significantly high expression of CALD1 in glioma using quantitative real-time polymerase chain reaction (PCR) and Western blotting. Meanwhile, we also conducted relevant cell experiments to prove that CALD1 can affect the proliferation and migration ability of glioma cells in vitro.
Conclusions:
Our results confirmed that CALD1 may be a prognostic marker for glioma and a potential target for immunotherapy in the future.
Insights
Caldes1 (CALD1) promotes glioma development by influencing M2 macrophage infiltration and is linked to patient survival. This study identifies CALD1 as a potential prognostic marker and therapeutic target for glioma immunotherapy.
Area of Science:
- Oncology
- Immunology
- Genetics
Background:
- Caldes1 (CALD1) is implicated in various tumor microenvironments (TME) and patient survival.
- Limited research exists on CALD1's role in the glioma immune microenvironment.
- CALD1's association with glioma pathogenesis and progression warrants investigation for novel therapeutic targets.
Purpose of the Study:
- To investigate the correlation between CALD1 and glioma pathogenesis and progression.
- To explore CALD1's role in the glioma immune microenvironment.
- To identify CALD1 as a potential therapeutic target for glioma.
Main Methods:
- Bioinformatic analysis of CALD1 in pan-cancer and glioma TME.
- Experimental verification including quantitative real-time PCR and Western blotting.
- In vitro cell experiments assessing glioma cell proliferation and migration.
Main Results:
- CALD1 expression in glioma correlates with infiltrating immune cells, particularly M2 macrophages.
- CALD1 is associated with tumor mutation burden, microsatellite instability, copy number variation, methylation, and stem cell index.
- CALD1 expression correlates with overall survival, progression-free interval, and disease-specific survival across multiple tumor types.
Conclusions:
- CALD1 is significantly upregulated in glioma and promotes its development.
- CALD1 serves as a potential prognostic marker for glioma.
- CALD1 represents a promising target for future glioma immunotherapy.
