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On-axis full-field swept-source optical coherence tomography for murine retinal imaging.

Ratheesh K Meleppat, Denise Valente, Soohyun Lee

|August 15, 2024

Summary

A novel full-field swept-source optical coherence tomography (FF-SS-OCT) system enables high-resolution in vivo imaging of the murine retina. This advanced OCT technology reveals detailed retinal structures and vascular plexuses for enhanced preclinical research.

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Area of Science:

Biomedical Optics
Ophthalmology
Preclinical Imaging

Background:

Optical Coherence Tomography (OCT) is crucial for retinal imaging.
Existing OCT systems face limitations in speed and resolution for in vivo murine studies.
Need for advanced imaging techniques to visualize microscopic retinal features.

Purpose of the Study:

To demonstrate a full-field swept-source OCT (FF-SS-OCT) system for in vivo murine retinal imaging.
To achieve high-speed, high-resolution imaging of retinal microstructures.
To provide a tool for detailed analysis of retinal layers and vascular networks.

Main Methods:

Development of an on-axis FF-SS-OCT system using a Mach-Zehnder interferometer.
Incorporation of a tunable laser source and a fast 2D-CMOS camera.
Optimization of imaging interface, spatial sampling, and pupil stop for enhanced image quality and aberration reduction.

Main Results:

Demonstration of FF-SS-OCT for in vivo murine retinal imaging at 150 Hz sweep rate.
Achieved effective axial scan rate of 20 million A-scans/s and field of view of 820 × 410 µm.
Obtained high-quality retinal B-scans and en face images revealing all major retinal layers and vascular plexuses.

Conclusions:

The developed FF-SS-OCT system provides high-speed, high-resolution imaging of the murine retina.
This technology is suitable for detailed visualization of retinal microanatomy and vasculature in preclinical research.
FF-SS-OCT offers a valuable tool for advancing ophthalmological research and drug development.