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Updated: Jun 27, 2026
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Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
Published on: June 25, 2014
Protocol for preparing mammalian skin samples encompassing hair follicles for spatial transcriptomics.
Yujia Jiang1, Ruikang Li2, Yixin Li3
1Department of Biology, University of Copenhagen, DK-2100 Copenhagen, Denmark; BGI Research, Hangzhou 310030, China.
STAR Protocols
|August 15, 2024
Summary
This study introduces a detailed protocol for spatial transcriptomics on mammalian skin, including hair follicles. This method allows for high-resolution gene expression analysis in skin tissue.
Area of Science:
- Molecular Biology
- Genomics
- Tissue Analysis
Background:
- Spatial transcriptomics offers single-cell resolution of gene expression in tissues.
- Analyzing skin tissue with spatial transcriptomics presents unique challenges.
Purpose of the Study:
- To develop and present a comprehensive protocol for preparing mammalian skin samples for spatial transcriptomics.
- To enable high-resolution gene expression analysis in skin, including structures like hair follicles.
Main Methods:
- Detailed steps for sample preparation, including embedding and cryosectioning.
- Procedures for RNA quality control, tissue fixation, staining, and imaging.
- Methods for sample permeabilization, reverse transcription, and cDNA collection.
Main Results:
- A robust protocol for spatial transcriptomics on mammalian skin samples has been established.
- The protocol successfully integrates sample preparation with gene expression analysis techniques.
Conclusions:
- This protocol facilitates advanced spatial transcriptomics studies in mammalian skin.
- It provides a foundation for investigating skin biology and disease at a high resolution.
