Related Experiment Video
Updated: Jun 16, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Fishing for obesity-related gut microbiome enterotype
Xuechen Rao1, Yikai Shao1, Hao Wu1
1Fudan Microbiome Center, State Key Laboratory of Genetic Engineering, School of Life Sciences, Human Phenome Institute, and Center for Obesity and Hernia Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
Abstract:
Despite debate, the concept of enterotype-like clusters remains valuable for exploring the human gut microbiome and associated environmental factors. In this issue of Cell Host & Microbe, Wu et al. robustly identified an obesity-related enterotype-like cluster, Megamonas, and demonstrated its clinical relevance through cohort studies, mice, and cell experiments.
Related Concept Videos
Obesity
Anatomy of the Intestines
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
What is Monogastric Digestion?
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Irritable Bowel Syndrome I: Introduction
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...

