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Cell-mediated Immune Responses

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Updated: Jun 16, 2025

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Trained immunity suppression determines kidney allograft survival.

Inge Jonkman¹, Maaike M E Jacobs¹, Yutaka Negishi²

¹Department of Nephrology, Radboud University Medical Center, Nijmegen, The Netherlands.

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons

|August 15, 2024

Summary

Post-transplant serum from kidney recipients can suppress trained immunity, a key factor in immune response. This suppression is linked to better long-term kidney graft survival, suggesting a new therapeutic target.

Keywords:

graft survival innate immunity kidney transplantation trained immunity

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Last Updated: Jun 16, 2025

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

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Published on: August 12, 2017

Mouse Kidney Transplantation: Models of Allograft Rejection

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Published on: October 11, 2014

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

Published on: May 2, 2013

Area of Science:

Immunology
Transplantation immunology
Innate immunity

Background:

The innate immune system is crucial for kidney transplant outcomes, but its precise role in long-term graft survival remains incompletely understood.
Trained immunity, characterized by epigenetic and metabolic reprogramming of innate immune cells, enhances inflammatory responses to subsequent stimuli.
Understanding how innate immune cells influence kidney allograft survival is critical for improving patient outcomes.

Purpose of the Study:

To investigate the role of trained immunity in kidney allograft survival.
To determine the impact of post-transplant serum from kidney transplant recipients on trained immunity.
To explore the potential of trained immunity as a therapeutic target for enhancing kidney graft survival.

Main Methods:

Peripheral blood mononuclear cells from healthy donors were stimulated in vitro to assess trained immunity.
Stimulation involved using pre-transplant and post-transplant serum from kidney transplant patients and immunosuppressive drugs.
Tumor necrosis factor and interleukin 6 cytokine levels were measured as readouts for trained immunity.

Main Results:

Serum collected from kidney transplant recipients one week post-transplantation demonstrated suppression of trained immunity.
A strong correlation was observed between the degree of trained immunity suppression by post-transplant serum and reduced rates of graft loss.
The suppressive effects of post-transplant serum are potentially mediated by novel actions of immunosuppressive drugs.

Conclusions:

Post-transplant serum possesses the capacity to suppress trained immunity in kidney transplant recipients.
Suppression of trained immunity by post-transplant serum is a significant predictor of long-term kidney graft survival.
Trained immunity emerges as a promising therapeutic target for optimizing immunosuppression and improving kidney transplant outcomes.