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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Related Experiment Video

Updated: Jun 16, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

An image processing pipeline for electron cryo-tomography in RELION-5.

Alister Burt^1,2, Bogdan Toader¹, Rangana Warshamanage^3,4

¹MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.

|August 15, 2024

Summary

This summary is machine-generated.

We developed a new image processing pipeline in RELION-5 for electron tomography. This tool standardizes data analysis from raw movies to atomic model building, improving macromolecular complex structure determination.

Keywords:

electron microscopy electron tomography image processing software

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Last Updated: Jun 16, 2025

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Area of Science:

Structural biology
Cryo-electron tomography
Macromolecular complex analysis

Background:

Electron tomography enables macromolecular complex structure determination in native environments.
Current image processing lacks standardization, hindering new researchers.
High-resolution structures are achievable through averaging noisy tomographic reconstructions.

Purpose of the Study:

To present a standardized image processing pipeline for electron tomography data in RELION-5.
To facilitate structure determination of macromolecular complexes.
To enhance interoperability and accessibility for researchers in the field.

Main Methods:

Development of a comprehensive image processing pipeline within RELION-5.
Inclusion of functionalities from raw movie import to automated atomic model building.
Explicit definition of metadata for improved interoperability.

Main Results:

A complete pipeline for electron tomography data processing is now available.
The pipeline supports automated atomic model building from tomographic reconstructions.
Standardized metadata facilitates integration with other software tools.

Conclusions:

The RELION-5 pipeline offers a standardized approach to electron tomography data analysis.
This work lowers the barrier to entry for researchers studying macromolecular complexes.
The defined metadata framework promotes future standardization in the field.