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Interlaboratory comparison of phosphatidylethanol in dried blood spots using different sampling devices
Matthias Bantle1,2, Frederike Stöth1, Wolfgang Weinmann1
1Institute of Forensic Medicine, Forensic Toxicology and Chemistry, University of Bern, Bern, Switzerland.
Drug Testing and Analysis
|August 16, 2024
Summary
Phosphatidylethanol (PEth) testing for monitoring alcohol consumption shows good interlaboratory comparability. Most labs accurately quantified PEth levels, supporting its use as a reliable biomarker for drinking behavior and abstinence.
Area of Science:
- Clinical Chemistry
- Forensic Toxicology
- Biomarker Analysis
Background:
- Phosphatidylethanol (PEth) is a key biomarker for assessing alcohol consumption and monitoring abstinence.
- Limited knowledge exists regarding the robustness, standardization, and comparability of PEth measurement methods.
- The first international consensus on PEth use and interpretation was published in 2022.
Purpose of the Study:
- To establish an experience-based foundation for harmonizing PEth testing methods.
- To assess the interlaboratory comparability of PEth quantification using microsamples.
- To evaluate the performance of laboratories in reporting PEth results.
Main Methods:
- Three rounds of interlaboratory comparison studies were conducted using microsamples.
- Participating laboratories applied authentic blood samples to their own devices.
- PEth 16:0/18:1 concentrations ranged from 16 to 474 ng/mL, including levels near decision limits.
Main Results:
- 73% of laboratories accurately quantified and reported all tested samples within acceptable limits.
- Over 90% of laboratories successfully quantified and reported at least one sample within acceptable limits.
- Results were evaluated using guidelines from Horwitz and the GTFCh.
Conclusions:
- Interlaboratory comparison demonstrates good comparability for PEth quantification.
- The study provides a foundation for further harmonization of PEth testing methods.
- PEth testing is a robust method for assessing drinking behavior and monitoring abstinence.
