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Updated: Jun 16, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Analysis of Intestinal Microbiota in Schizophrenic Patients with Type 2 Diabetes Mellitus
Nan Wang1,2, Chunlei Yin2,3, Ruiqi Feng4
1Department of Medicine, Dali University, Dali, China.
Objective:
Our goal is to examine the correlation between gut microbiota and the cooccurrence of schizophrenia and type 2 diabetes.
Methods:
We conducted a study on the intestinal microbiota of 4 distinct groups: simple schizophrenia group (SC), schizophrenia with type 2 diabetes group (TS), type 2 diabetes group (T2DM), and normal population control group (HC), comprising a total of 35 subjects.
Results:
The bacteria phyla Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Verrucobacteria were consistently present across all 4 groups. Significantly higher intestinal microbiota richness was observed in the T2DM compared to the other group, and the intestinal microbiota richness in TS significantly lower than that of the SC.
Conclusion:
Our study suggests that the presence of type 2 diabetes in individuals with schizophrenia may affect the composition of their gut microbiota. We hypothesize that the concurrent existence of both diseases could potentially lead to alterations in the structure of gut microbiota, potentially influencing treatment effectiveness and outcomes.
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