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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Jun 16, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Multi-species cryoEM calibration and workflow verification standard.

Daija Bobe¹, Mykhailo Kopylov¹, Jessalyn Miller¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA.

Biorxiv : the Preprint Server for Biology

|August 16, 2024

Summary

Researchers now have a new calibration standard for cryogenic electron microscopy (cryoEM). This test sample helps optimize resolution and evaluate instrument performance for structural biology.

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Last Updated: Jun 16, 2025

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Area of Science:

Structural biology
Biophysics

Background:

Cryogenic electron microscopy (cryoEM) is a powerful technique for determining molecular structures.
Established methods often lack standardized calibration samples, hindering performance evaluation.

Purpose of the Study:

To introduce a novel cryoEM calibration sample.
To provide a standardized tool for validating cryoEM pipelines and methodologies.

Main Methods:

Developed a mixture of compatible macromolecules as a single test specimen.
Designed the sample for resolution optimization and multi-point performance evaluation.

Main Results:

The calibration sample enables simultaneous assessment of instrument performance, data quality, and image processing workflows.
Provides reference points for validating and benchmarking cryoEM techniques.

Conclusions:

This cryoEM calibration sample addresses the need for standardized biological test specimens.
Facilitates reliable validation and benchmarking of cryoEM data acquisition and analysis.