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Updated: Jun 16, 2025
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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
Published on: October 28, 2021
9.0K
Chalcones induce apoptosis, autophagy and reduce spreading in osteosarcoma 3D models
M Rossi1, C Pellegrino2, M M Rydzyk1
1Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, Bologna 40127, Italy; Center for Applied Biomedical Research (CRBA), Alma Mater Studiorum University of Bologna, Bologna 40126, Italy.
Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|August 16, 2024
Summary
Natural compounds Licochalcone A (Lic-A) and its derivatives show promise against osteosarcoma. These chalcones induce apoptosis and autophagy, inhibiting cancer cell spread and offering potential new treatments for this bone cancer.
Area of Science:
- Oncology
- Natural Product Chemistry
- Molecular Biology
Background:
- Osteosarcoma is a prevalent bone cancer with poor prognosis due to metastasis.
- Current treatments are limited by incomplete understanding and lack of effective in vitro models.
- Chalcones, including Licochalcone A (Lic-A), are natural compounds with potential therapeutic value.
Purpose of the Study:
- To evaluate the anticancer effects of Lic-A and its derivatives on osteosarcoma.
- To investigate the mechanism of action of promising chalcone derivatives.
- To assess the potential of chalcones as antiproliferative agents for osteosarcoma.
Main Methods:
- Utilized multicellular tumor spheroids (MCTSs) from MG63 and 143B osteosarcoma cell lines.
- Assessed metabolic activity to identify potent compounds.
- Conducted caspase activity assays and invasion assays to determine mechanisms of action.
Main Results:
- Lic-A, 1i, and 1k derivatives demonstrated significant anticancer activity.
- Lic-A and 1k induced apoptosis and autophagy in osteosarcoma cells.
- Lic-A and 1k reduced the invasive potential of osteosarcoma cells.
Conclusions:
- Chalcones are effective natural scaffolds for developing osteosarcoma treatments.
- Lic-A and 1k show significant antiproliferative effects by inducing apoptosis and autophagy.
- Lic-A and 1k represent promising candidates for novel osteosarcoma therapies.
