Triage performance of PAX1^m/JAM3^m in opportunistic cervical cancer screening of non‒16/18 human...

In this study, we aimed to validate the performance of the PAX1 and JAM3 methylation (PAX1^m/JAM3^m) test as a triage tool for detecting cervical intraepithelial neoplasia grade 3 or worse (CIN3 +) in non-16/18 high-risk human papillomavirus-positive patients (non-16/18 hrHPV +).

The triage performance of liquid-based cytology (LBC) and the PAX1^m/JAM3^m test for detecting CIN3 + were compared.

In total, 1851 participants had cervical histological outcomes and were included in the analysis. The sensitivity/specificity of the LBC test results with atypical squamous cells of undetermined significance or worse (LBC ≥ ASCUS) and the PAX1^m/JAM3^m test were 90.1%/26.7% and 84.8%/88.5%, respectively. PAX1^m/JAM3^m( +) had the highest diagnostic AUC (0.866, 95% confidence interval (CI) 0.837-0.896) in the whole cohort. All cancers (n = 20) were detected by PAX1^m/JAM3^m(+). Compared with LBC ≥ ASCUS, PAX1^m/JAM3^m(+) reduced the number of patients who needed referral for colposcopy by 57.21% (74.66% vs. 17.45%). The odds ratios for detecting CIN3 + by LBC ≥ ASCUS and PAX1^m/JAM3^m(+) were 3.3 (95% CI 2.0-5.9) and 42.6 (27.1-69.6), respectively (p < 0.001). The combination of LBC ≥ ASCUS or PAX1^m/JAM3^m(+) slightly increased the diagnostic sensitivity (98.0%, 95% CI: 95.8-100%) and referral rate (77.09%) but reduced the diagnostic specificity (24.8%, 22.7-26.8%).

In non-16/18 hrHPV(+) women, PAX1^m/JAM3^m was superior to cytology for detecting CIN3 + . Compared with LBC ≥ ASCUS, PAX1^m/JAM3^m(+) reduced the number of significant referrals to colposcopy without compromising diagnostic sensitivity.