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Relationship Between Cryoprotectant Potential and Protein Hydration in Aqueous Zwitterionic Solutions
Takahiro Takekiyo1, Shuto Yamada1, Tetsuya Hirata2
1Department of Applied Chemistry, National Defense Academy, Yokosuka, Kanagawa, Japan.
Biopolymers
|August 17, 2024
Summary
The cryoprotectant 3-(1-(2-(2-methoxyethoxy)ethyl)imidazol-3-io)butane-1-carboxylate (OE2imC3C) shows higher protein stability at a mole fraction of 1.7×10-2. This indicates OE2imC3C
Area of Science:
- Biochemistry
- Materials Science
Background:
- Protein stability is crucial for cryopreservation.
- Understanding cryoprotectant-protein interactions is key to improving preservation methods.
Purpose of the Study:
- To assess the cryoprotectant potential of 3-(1-(2-(2-methoxyethoxy)ethyl)imidazol-3-io)butane-1-carboxylate (OE2imC3C) for proteins.
- To elucidate the relationship between OE2imC3C concentration and protein hydration and stability.
Main Methods:
- Fourier-transform infrared (FTIR) spectroscopy.
- Near-UV circular dichroism (near-UV CD) spectroscopy.
- Differential scanning calorimetry (DSC).
Main Results:
- Protein stability was preserved in OE2imC3C solutions at a mole fraction of 1.7×10-2 after freezing and thawing.
- Protein stability was reduced at a mole fraction of 7.7×10-3.
- Preferential binding of OE2imC3C to proteins was observed.
Conclusions:
- OE2imC3C demonstrates cryoprotective properties for proteins.
- Higher concentrations of OE2imC3C (1.7×10-2) enhance protein stability more effectively than lower concentrations (7.7×10-3).
- The cryoprotectant potential is linked to the preferential interaction between OE2imC3C and proteins.
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