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Simultaneous Detection of Biomarkers in Urine Using a Multicalibration Potentiometric Sensing Array Combined with a
Shengqi Cheng1, Fan Wang2, Shaohua Zuo3
1School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, People's Republic of China.
Analytical Chemistry
|August 17, 2024
Summary
A novel 10-channel potentiometric sensing array enables simultaneous detection of uric acid, creatinine, and urea in urine. This device offers accurate, real-time health monitoring for early disease detection.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Sensor Technology
Background:
- Uric acid (UA), creatinine, and urea in urine are key biomarkers for diagnosing kidney diseases.
- Domestic health monitoring devices are crucial for regular and real-time tracking of health status.
- Accurate and simultaneous detection of multiple urine biomarkers is essential for effective disease management.
Purpose of the Study:
- To develop a multi-analyte sensing array for simultaneous detection of UA, creatinine, urea, and pH in urine.
- To integrate calibration channels and temperature/pH compensation for enhanced monitoring accuracy.
- To validate the performance of the developed system against commercial diagnostic tools for practical application.
Main Methods:
- Fabrication of a 10-channel potentiometric sensing array with specific electrode groups for UA, creatinine, urea, and pH.
- Utilization of functionalized sensing materials including Pt/carbon nanotubes (CNTs)-uricase, creatinine deiminase, Au@urease, and polyaniline.
- Integration of calibration channels and external probes for pH and temperature compensation.
- Development of a portable potentiometric analyzer for data processing and direct result display.
Main Results:
- The sensing array demonstrated high sensitivity and selectivity for simultaneous detection of UA, creatinine, urea, and pH.
- Integrated calibration and compensation methods significantly improved the accuracy of domestic monitoring.
- The developed system showed excellent agreement with results from commercial kits and pH meters.
- The analyzer successfully displayed direct concentration values for the target analytes and pH in Practical Mode.
Conclusions:
- The proposed potentiometric sensing array is highly suitable for developing advanced domestic health monitoring devices.
- The system offers a reliable and accurate method for simultaneous urine biomarker analysis, facilitating early disease detection.
- This technology holds significant potential for improving patient self-management and healthcare accessibility.
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