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Related Concept Videos

Archival Research01:40

Archival Research

Some researchers gain access to large amounts of data without interacting with a single research participant. Instead, they use existing records to answer various research questions. This type of research approach is known as archival research. Archival research relies on looking at past records or data sets to look for interesting patterns or relationships. For example, a researcher might access the academic records of all individuals who enrolled in college within the past ten years and...
Bioavailability Study Design: Healthy Subjects Versus Patients01:15

Bioavailability Study Design: Healthy Subjects Versus Patients

Bioavailability studies are essential for evaluating a drug's therapeutic efficacy and understanding its absorption patterns under various physiological conditions. Conducting such studies on target patient populations provides more relevant data by simulating real-world disease states. However, practical challenges often necessitate the use of young, healthy adult volunteers as study subjects.Patients may exhibit altered drug absorption patterns due to the effects of the disease itself,...

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Establishment of a Clinic-based Biorepository
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Using established biorepositories for emerging research questions: a feasibility study.

Lente J S Lerink1,2, Christopher W Sutton3, Henny G Otten

  • 1Nuffield Department of Surgical Sciences, University of Oxford, Oxford, UK.

Clinical Proteomics
|August 17, 2024
PubMed
Summary

Long-term stored clinical serum samples are suitable for proteomic and metabolomic pathway analysis. This finding supports future research in kidney transplantation and rare disease studies.

Keywords:
BiobankKidney transplantationMetabolomicsPlasmaProteomicsSerum

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Area of Science:

  • Biomolecular analysis
  • Transplant research
  • Biobanking

Background:

  • Kidney transplant research can benefit from proteomics and metabolomics.
  • Donor samples are available through biobanks, but recipient samples for long-term studies are lacking.
  • Clinical serum repositories hold potential but require validation for -omics applications due to non-uniform handling.

Purpose of the Study:

  • To assess the suitability of long-term stored clinical serum samples for proteomic and metabolomic pathway analysis.
  • To compare clinical serum samples with protocolized biobank samples.

Main Methods:

  • Mass spectrometry-based shotgun proteomics and metabolomics were employed.
  • Clinical serum samples from the PROCARE consortium were analyzed.
  • Parallel analysis was conducted on samples from the QUOD biobank.

Main Results:

  • Over 800 compounds and 600 proteins were identified in both sample types.
  • 163 endogenous metabolites were common to both clinical and biobanked samples.
  • Integrated pathway analysis successfully mapped proteomic and metabolomic data, enriching 79 pathways.

Conclusions:

  • Long-term stored clinical serum samples are viable for qualitative proteomic and metabolomic pathway profiling.
  • This validates the use of existing clinical samples for future research.
  • Implications include advancing biomedical research, long-term outcome studies, and rare event investigations.