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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Published on: June 23, 2013
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Protocol for analyzing BCG-induced trained immunity in murine bone marrow-derived macrophages
Jin-Chuan Xu1, Zhidong Hu1, Xiao-Yong Fan1
1Shanghai Institute of Infectious Diseases and Biosecurity & Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
STAR Protocols
|August 18, 2024
Summary
This study details a protocol for analyzing Bacillus Calmette-Guérin (BCG)-induced trained immunity in mouse macrophages. This method aids researchers in studying innate immune memory and its role in vaccine-mediated protection.
Area of Science:
- Immunology
- Vaccinology
- Cell Biology
Background:
- Bacillus Calmette-Guérin (BCG) is the sole licensed tuberculosis vaccine.
- BCG confers non-specific protection against various diseases via trained immunity.
- Trained immunity involves functional reprogramming of the innate immune system through immune memory.
Purpose of the Study:
- To present a detailed protocol for analyzing BCG-induced trained immunity.
- To enable researchers to utilize murine bone marrow-derived macrophages (BMDMs) for studying trained immunity.
- To facilitate research into the mechanisms of innate immune memory.
Main Methods:
- Preparation of BCG single bacterial suspensions.
- Isolation of murine bone marrow-derived macrophages (BMDMs).
- Induction of trained immunity in BMDMs using BCG.
Main Results:
- A reproducible protocol for studying BCG-induced trained immunity in BMDMs is established.
- The protocol outlines key steps from bacterial preparation to cell training.
- This method provides a convenient approach for investigating trained immunity.
Conclusions:
- The presented protocol offers a standardized method for analyzing BCG-induced trained immunity in BMDMs.
- This research tool can advance the understanding of innate immune memory.
- The protocol supports further investigation into the non-specific protective effects of BCG vaccination.
