Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Vitamins in Maintaining Bone Health01:25

Role of Vitamins in Maintaining Bone Health

3.2K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.2K
Bone Remodeling01:40

Bone Remodeling

38.2K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.2K
Bone Disorders01:29

Bone Disorders

3.4K
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
3.4K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

2.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.9K
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

2.6K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.6K
Management of Insomnia01:19

Management of Insomnia

237
The sleep cycle, an integral part of human health, consists of several stages with distinct characteristics and functions. It begins with a transition from wakefulness to sleep, known as the light sleep phase, followed by the restorative deep sleep phase, essential for physical recovery and growth. The cycle concludes with the Rapid Eye Movement (REM) phase, characterized by high brain activity and vivid dreaming. Insomnia, a prevalent sleep disorder, involves difficulty falling asleep, staying...
237
  1. Home
  3. Research Domains
  5. Indigenous Studies
  7. Te Hauora Me Te Oranga O Te Māori (māori Health And Wellbeing)
  9. Te Ariā O Ngā Tauira Panoni Hauora O Te Māori (māori Theory Of Change Models For Health)
  11. Melatonin Prevents Bone Loss In Osteoporotic Rats With Valproic Acid Treatment By Anti-inflammatory And Anti-oxidative Stress

Melatonin prevents bone loss in osteoporotic rats with valproic acid treatment by anti-inflammatory and anti-oxidative stress

Zhou-Shan Tao1, Xu-Feng Hu2, Tao Sun3

  • 1Department of Orthopedics, The First Affiliated Hospital of Wannan Medical College, Yijishan Hospital, No. 2, Zhe Shan Xi Road, Wuhu 241001, Anhui, PR China; Anhui Province Key Laboratory of Non-coding RNA Basic and Clinical Transformation, No. 2, Zhe Shan Xi Road, Wuhu 241001, Anhui, PR China.

International Immunopharmacology
|August 18, 2024

Related Experiment Videos

Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
08:56

Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats

Published on: April 7, 2023

851
Author Spotlight: Exploring the Impact of Lingnan Fire-Needle Therapy in Osteoporosis Intervention
03:56

Author Spotlight: Exploring the Impact of Lingnan Fire-Needle Therapy in Osteoporosis Intervention

Published on: April 26, 2024

558
Author Spotlight: Integrating Traditional Chinese Medicine with Modern Pharmacology and Genomics for Assessing Postmenopausal Osteoporosis in Mice
07:20

Author Spotlight: Integrating Traditional Chinese Medicine with Modern Pharmacology and Genomics for Assessing Postmenopausal Osteoporosis in Mice

Published on: August 23, 2024

468

View abstract on PubMed

Summary
This summary is machine-generated.

Melatonin (MEL) protects against valproic acid (VPA)-induced bone loss by reducing inflammation and oxidative stress. This study shows MEL enhances bone formation and inhibits bone resorption, improving bone metabolism and strength in VPA-treated rats.

Area of Science:

  • Biomedical research
  • Pharmacology
  • Bone biology

Background:

  • Melatonin (MEL) exhibits anti-inflammatory and antioxidant properties.
  • Valproic acid (VPA) can induce bone loss.
  • The impact of MEL on VPA-induced bone loss requires investigation.

Purpose of the Study:

  • To investigate the protective effects of melatonin against valproic acid-induced bone loss in a rat model.
  • To elucidate the mechanisms underlying melatonin's action on bone metabolism.

Main Methods:

  • In vitro studies using MC3T3-E1 and RAW264.7 cells to assess osteogenic and osteoclastic differentiation.
  • In vivo experiments in VPA-treated rats to evaluate bone mineral density, bone strength, and relevant gene/protein expression.
  • Assays included CCK-8, ALP staining, AR staining, and TRAP staining.
Keywords:
Bone massInflammationMelatoninOidative stress

Related Experiment Videos

Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
08:56

Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats

Published on: April 7, 2023

851
Author Spotlight: Exploring the Impact of Lingnan Fire-Needle Therapy in Osteoporosis Intervention
03:56

Author Spotlight: Exploring the Impact of Lingnan Fire-Needle Therapy in Osteoporosis Intervention

Published on: April 26, 2024

558
Author Spotlight: Integrating Traditional Chinese Medicine with Modern Pharmacology and Genomics for Assessing Postmenopausal Osteoporosis in Mice
07:20

Author Spotlight: Integrating Traditional Chinese Medicine with Modern Pharmacology and Genomics for Assessing Postmenopausal Osteoporosis in Mice

Published on: August 23, 2024

468

Main Results:

  • VPA impaired osteogenic differentiation and promoted osteoclast differentiation, associated with increased inflammation and oxidative stress.
  • Melatonin treatment reversed VPA-induced effects, boosting osteogenesis and inhibiting osteoclast differentiation.
  • In vivo, MEL increased SOD2 and decreased TNF-α expression, restoring bone metabolism and improving bone mineral density and strength.

Conclusions:

  • Melatonin effectively alleviates valproic acid-induced bone loss in rats.
  • MEL exerts its protective effects by reducing oxidative stress and inflammation, enhancing osteogenic activity, and inhibiting osteoclast differentiation.
  • Melatonin shows therapeutic potential for managing VPA-induced bone loss.
Valproic acid