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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 16, 2025

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Detachable acoustofluidic droplet-sorter.

Dhrubajyoti Das¹, Shih-Hong Huang², Choa-Li Weng³

¹Department of Biomedical Engineering, National Cheng Kung University, Tainan, 701, Taiwan.

Analytica Chimica Acta

|August 18, 2024

Summary

A new acoustofluidic device sorts single cells with high purity and viability, overcoming limitations of fluorescence-activated cell sorting (FACS). This cost-effective technology offers a reliable alternative for precise cell isolation in research and clinical applications.

Keywords:

Acoustofluidic device Cell sorting Droplet IDT Microchip Surface acoustic wave

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Published on: June 20, 2020

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Cell sorting is vital for analyzing cell functions, disease diagnosis, drug discovery, and regenerative medicine.
Fluorescence-activated cell sorting (FACS) is effective but costly, complex, and can compromise cell integrity.
Existing methods necessitate improvements in cost-effectiveness, portability, and cell viability preservation.

Purpose of the Study:

To develop a novel acoustofluidic device for high-purity single-cell isolation.
To address the limitations of current cell sorting technologies, particularly FACS.
To enhance cell viability during the sorting process.

Main Methods:

Integration of surface acoustic wave (SAW) technology with droplet microfluidics.
Development of a detachable microfluidic chip design for reusability.
Utilizing human embryonic kidney cells transfected with fluorescent reporter plasmids for demonstration.

Main Results:

The acoustofluidic sorter achieved 81% recovery and >97% accuracy in isolating single-cell droplets.
Maintained a high cell viability rate of 95% throughout the sorting process.
Demonstrated consistent repeatability over 20 trials with stable operating temperatures.

Conclusions:

The developed acoustofluidic device is a sensitive and effective tool for single-cell sorting.
The reusable and detachable chip design offers a cost-effective solution, reducing cross-contamination risks.
This technology shows significant potential for advancing research and clinical applications requiring precise cell isolation.