The polyhedrin gene of Bombyx mori nuclear polyhedrosis virus

Cloned SalI fragments of Bombyx mori nuclear polyhedrosis virus (BmSNPV) DNA were screened with the polyhedrin gene of Autographa californica nuclear polyhedrosis virus as a probe. One positive clone, pBN61, with an insert of 1.65 Kb, was obtained. The HindIII, HpaII and AluI maps of the insert were constructed. Part of its nucleotide sequence has been determined. The 46 amino acid sequence, as determined from the nucleotide sequence, was compared with the reported sequence of BmSNPV polyhedrin. Only one amino acid difference has been found. It is likely that clone pBN61 contains the whole BmSNPV polyhedrin gene.