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Related Experiment Video

Updated: Jun 16, 2025

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

Enterococcal quorum-controlled protease alters phage infection.

Emma K Sheriff¹, Fernanda Salvato², Shelby E Andersen¹

¹Department of Immunology and Microbiology, School of Medicine, University of Colorado - Anschutz Medical Campus, 12800 E. 19th Ave., Aurora, CO 80045, United States.

|August 19, 2024

Summary

Bacteriophages (phages) show promise against multidrug-resistant bacteria. This study reveals how phage VPE25 alters Enterococcus faecalis virulence factors like GelE, offering insights for phage therapy development.

Keywords:

Enterococcus bacteriophage phage–host interactions protease proteomics quorum sensing

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Last Updated: Jun 16, 2025

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

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Published on: August 19, 2021

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Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

Area of Science:

Microbiology
Virology
Proteomics

Background:

Rising multidrug-resistant bacterial infections necessitate novel antimicrobial strategies.
Bacteriophages (phages) are promising alternatives, but their therapeutic application is limited by incomplete understanding of infection dynamics.

Purpose of the Study:

To elucidate the molecular mechanisms of phage VPE25 infection in Enterococcus faecalis.
To identify bacterial protein changes during phage infection and their impact on virulence.

Main Methods:

Proteomic analysis of E. faecalis infected with phage VPE25.
Characterization of bacterial protein abundance changes, focusing on enterococcal gelatinase (GelE).
Plaque assays on mutant strains (fsrA, gelE) to assess phage infection susceptibility.

Main Results:

Numerous uncharacterized phage proteins and hundreds of altered bacterial protein abundances were identified.
Enterococcal gelatinase (GelE) levels decreased during VPE25 infection.
Mutations in fsrA or gelE led to larger plaques with halo morphology, indicating reduced phage resistance.

Conclusions:

GelE plays a role in protecting E. faecalis from phage infection, potentially via LrgA and LrgB regulation.
Modulating GelE production could be a strategy to enhance phage therapy efficacy by reducing virulence and biofilm formation.