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Implementation of RSM and ANN Optimization Approach for Natural Deep Eutectic Solvents-Based Extraction of Bioactive
Rachna Gupta1,2, Anupama Singh1, Prabhat K Nema1
1Department of Food Engineering, National Institute of Food Technology, Entrepreneurship and Management (Institute of National Importance, Under MoFPI, Govt. of India), Kundli, District - Sonipat, Haryana 131028, India.
This study developed an eco-friendly method using natural deep eutectic solvents and microwave-assisted extraction to obtain bioactive compounds from orange peel. This approach enhances antioxidant activity and total phenolic and flavonoid content, offering a sustainable way to utilize citrus waste.
Area of Science:
- Green Chemistry
- Food Science
- Biotechnology
Background:
- Orange peel is a rich source of bioactive compounds.
- Valorization of food waste like orange peel is crucial for sustainability.
- Conventional extraction methods often lack efficiency and eco-friendliness.
Purpose of the Study:
- To develop an environmentally friendly method for extracting bioactive compounds from orange peel.
- To optimize the extraction process using natural deep eutectic solvents (NADES) and microwave-assisted extraction (MAE).
- To compare the efficiency of MAE with conventional heating-stirring extraction (HSE).
Main Methods:
- Extraction using NADES-II (choline chloride and ethylene glycol with water).
- Optimization of Microwave-Assisted Extraction (MAE) using Response Surface Methodology (RSM) and Artificial Neural Network (ANN).
- Comparison of MAE with conventional Heating-Stirring Extraction (HSE).
Main Results:
- NADES-II demonstrated maximal extraction yield and higher antioxidant activity (DPPH, ABTS), total phenolic content (TPC), and total flavonoid content (TFC).
- ANN provided a more accurate model for optimization compared to RSM.
- Optimized MAE conditions (13 min ramp, 52°C, 21 min hold, 20 mL/g ratio) yielded superior results over HSE.
Conclusions:
- MAE with NADES-II is an efficient and eco-friendly method for extracting bioactive compounds from orange peel.
- The optimized process significantly enhances antioxidant capacities, TPC, and TFC.
- This study presents a promising approach for the valorization of orange peel waste.
