Efficient and fast detection of uric acid based on a colorimetric sensing method

Area of Science:

• Biochemistry
• Analytical Chemistry
• Biomedical Diagnostics

Background:

• Uric acid (UA) levels are critical physiological indicators, with abnormalities linked to various diseases.
• Current UA detection methods (chromatography, electrophoresis, electrochemistry) are often complex, costly, and require specialized equipment, limiting their use in resource-limited settings.

Purpose of the Study:

• To develop a portable, visualized, and cost-effective method for detecting uric acid concentration.
• To overcome the limitations of traditional uric acid analysis methods, particularly in resource-limited environments.

Main Methods:

• Development of a detection method utilizing hydrogen peroxide (H2O2) test strips.
• Employing uric acid enzyme to catalyze the oxidation of uric acid, producing H2O2.
• Visualizing uric acid concentration through color changes on the H2O2 test strip.

Main Results:

• The developed method demonstrates good linearity in the range of 1–50 μg mL⁻¹ for uric acid detection.
• The assay provides a significant color change on the test strip proportional to H2O2 production.
• Successful development of a portable and visualized bioassay for uric acid.

Conclusions:

• The H2O2 test strip method offers a simple, rapid, and cost-effective approach for uric acid detection.
• This visualized bioassay is suitable for widespread application, especially in resource-limited areas.
• The method facilitates easier diagnosis of uric acid-related diseases.