Related Experiment Video
Updated: Jun 16, 2025
04:11
Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
13.1K
Hypoxia represses pattern-triggered immune responses in Arabidopsis
Brian C Mooney1, Catherine M Doorly1,2, Melissa Mantz3,4
1Department of Biology, Maynooth University, Maynooth, County Kildare, W23 F2H6, Ireland.
Plant Physiology
|August 19, 2024
Summary
Plants coordinate combined stress responses by regulating protein degradation. Hypoxia, a key abiotic stress, represses plant immune responses to biotic triggers, similar to animals.
Area of Science:
- Plant biology
- Molecular plant pathology
- Stress physiology
Background:
- Plants face concurrent biotic and abiotic stresses, necessitating coordinated responses.
- The ubiquitin/proteasome system, particularly the Arg/N-degron pathway, regulates stress responses via protein degradation.
- ETHYLENE RESPONSE FACTOR-VII (ERF-VII) transcription factors are crucial for hypoxia response.
Purpose of the Study:
- Investigate the role of the Arabidopsis Arg/N-degron pathway in mediating crosstalk between combined abiotic (hypoxia) and biotic (flg22-induced immunity) stresses.
- Elucidate the mechanisms underlying plant responses to co-occurring stresses.
Main Methods:
- Utilized hypoxia treatments and the flg22 elicitor in Arabidopsis thaliana.
- Analyzed transcriptional responses and key components of pattern-triggered immunity (PTI).
- Assessed the involvement of ERF-VIIs in the observed stress crosstalk.
Main Results:
- A link was identified between plant transcriptional responses to hypoxia and flg22.
- Hypoxia was found to repress the flg22 transcriptional program and PTI components, including pattern recognition receptors and MAPK signaling.
- This repression occurred largely independently of ERF-VIIs.
Conclusions:
- Hypoxia negatively impacts plant innate immunity, mirroring effects observed in animals.
- Understanding these stress tradeoffs is vital for enhancing crop resilience to climate change.
- The Arg/N-degron pathway's role in stress crosstalk warrants further investigation.
Related Concept Videos
02:41Responses to Drought and Flooding
10.6K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.6K
01:23Hypoxia
985
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
985
02:45Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K
01:10Transcription
146.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
146.8K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K