Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

3.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.5K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. The Impact Of Oleuropein On Cisplatin-induced Toxicity In Cochlear Cells In Relation To The Expression Of Deoxyribonucleic Acid Damage-associated Genes.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. The Impact Of Oleuropein On Cisplatin-induced Toxicity In Cochlear Cells In Relation To The Expression Of Deoxyribonucleic Acid Damage-associated Genes.

Related Experiment Video

Trans-Tympanic Drug Delivery for the Treatment of Ototoxicity
09:52

Trans-Tympanic Drug Delivery for the Treatment of Ototoxicity

Published on: March 16, 2018

9.4K

The Impact of Oleuropein on Cisplatin-Induced Toxicity in Cochlear Cells in Relation to the Expression of Deoxyribonucleic Acid Damage-Associated Genes.

Yüksel Olgun1, Zekiye Altun2, Merve Tütüncü2

  • 1Department of Otorhinolaryngology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey.

The Journal of International Advanced Otology
|August 19, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

More Related Videos

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
11:13

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

Published on: March 28, 2012

23.8K
The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
12:21

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

17.3K

Related Experiment Videos

Trans-Tympanic Drug Delivery for the Treatment of Ototoxicity
09:52

Trans-Tympanic Drug Delivery for the Treatment of Ototoxicity

Published on: March 16, 2018

9.4K
Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
11:13

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

Published on: March 28, 2012

23.8K
The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
12:21

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

17.3K

Oleuropein (OLE) protects cochlear cells from cisplatin (CDDP)-induced ototoxicity. OLE treatment altered DNA damage and repair gene expression, mitigating CDDP

Area of Science:

  • Ototoxicity research
  • Molecular biology of DNA damage and repair

Background:

  • Cisplatin (CDDP) chemotherapy can cause ototoxicity, damaging cochlear cells.
  • Oleuropein (OLE), a natural antioxidant, is investigated for protective properties.
  • Understanding gene expression changes in cochlear cells exposed to CDDP is crucial.

Purpose of the Study:

  • To evaluate the protective effect of Oleuropein (OLE) against Cisplatin (CDDP)-induced ototoxicity.
  • To analyze alterations in DNA damage and repair gene expression in cochlear cells.

Main Methods:

  • House Ear Institute-Organ of Corti 1 (HEI-OC1) cells were treated with CDDP, OLE, and OLE-CDDP.
  • Cell viability was assessed using the water-soluble tetrazolium salt assay.
  • DNA damage was quantified via flow cytometry, and gene expression changes were analyzed using a 84-gene reverse transcription polymerase chain reaction array.

Main Results:

  • CDDP significantly reduced HEI-OC1 cell viability, which was restored by OLE-CDDP treatment.
  • CDDP increased DNA damage markers (H2AX ratio), while OLE-CDDP decreased them.
  • OLE-CDDP modulated the expression of DNA damage and repair genes, downregulating damage-associated genes and upregulating repair genes (Abl1, Dbd2, Rad52, Trp53).

Conclusions:

  • Oleuropein (OLE) demonstrates a potential protective effect against Cisplatin (CDDP)-induced ototoxicity in cochlear cells.
  • OLE mitigates CDDP-induced ototoxicity by altering the expression profile of DNA damage and repair-related genes.
  • This study highlights OLE as a potential therapeutic agent for preventing chemotherapy-induced hearing loss.