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Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

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EFFECT OF ARTESUNATE TREATMENT ON INTESTINAL INJURY AFTER CARDIOPULMONARY RESUSCITATION IN SWINE.

Qianqian Wang¹, Peng Shen¹, Jiangang Zhu¹

¹Department of Intensive Care Unit, The First Hospital of Jiaxing, Jiaxing, China.

Shock (Augusta, Ga.)

|August 19, 2024

Summary

Artesunate (Art) treatment significantly reduces intestinal injury following cardiac arrest and resuscitation in swine. This protective effect is linked to the inhibition of pyroptosis, a key inflammatory pathway.

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Standardized Model of Ventricular Fibrillation and Advanced Cardiac Life Support in Swine

Standardized Model of Ventricular Fibrillation and Advanced Cardiac Life Support in Swine

Published on: January 30, 2020

Area of Science:

Cardiovascular Research
Gastroenterology
Pharmacology

Background:

Systemic ischemia-reperfusion injury post-cardiac arrest (CA) frequently causes intestinal damage.
Artesunate (Art) demonstrates protective effects against regional ischemia-reperfusion (I/R) injury in various organs.

Purpose of the Study:

To investigate the therapeutic potential of Artesunate (Art) in mitigating intestinal injury after CA and cardiopulmonary resuscitation (CPR) in a swine model.
To elucidate the underlying mechanisms of Art's protective effects on the intestine.

Main Methods:

A swine model of CA/CPR was established, followed by induction of ventricular fibrillation (VF) and CPR.
Artesunate (4.8 mg/kg) was administered intravenously to the treatment group post-resuscitation.
Intestinal injury markers, including biomarkers, tight junction proteins, apoptosis, pyroptosis proteins (caspase-3/GSDME), and cytokines, were assessed at various time points.

Main Results:

Artesunate treatment significantly improved intestinal injury biomarkers compared to the untreated CA/CPR group.
Art administration led to increased levels of intestinal zonula occluden-1 (ZO-1) and occludin.
The treatment group showed significantly reduced intestinal apoptosis and lower concentrations of pyroptosis proteins and proinflammatory cytokines.

Conclusions:

Artesunate effectively alleviates intestinal injury in a swine model of CA and CPR.
The protective mechanism of Artesunate may involve the inhibition of the caspase-3/GSDME-mediated pyroptosis pathway.
These findings suggest Artesunate as a potential therapeutic agent for post-cardiac arrest care.