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Related Concept Videos

CRISPR01:59

CRISPR

50.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Jun 16, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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Versatile plant genome engineering using anti-CRISPR-Cas12a systems.

Yao He1,2, Shishi Liu2,3, Long Chen4,5

  • 1Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Chongqing Key Laboratory of Tree Germplasm Innovation and Utilization, School of Life Sciences, Southwest University, Chongqing, 400715, China.

Science China. Life Sciences
|August 19, 2024
PubMed
Summary

Scientists established an anti-CRISPR-Cas12a system for plants, using AcrVA1 to control genome editing. This system reduces off-target mutations, fine-tunes editing efficiency, and enables inducible, tissue-specific editing and synthetic gene circuits.

Keywords:
AcrVA1CRISPRaCas12aanti-CRISPRfine-tuning genome editinginducible and tissue specific genome editingoff-target effectssynthetic logic circuit

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Area of Science:

  • Plant biotechnology
  • Genome engineering
  • Molecular biology

Background:

  • CRISPR-Cas12a systems are vital tools in plant research and crop improvement.
  • The application and efficacy of anti-CRISPR-Cas12a systems in plants remain underexplored.

Purpose of the Study:

  • To identify and characterize anti-CRISPR proteins for inhibiting Cas12a activity in plants.
  • To develop a versatile anti-CRISPR system for precise genome engineering in plants.

Main Methods:

  • In silico analysis to identify potential anti-CRISPR proteins.
  • In vitro and in planta assays to evaluate inhibition of Cas12a activity.
  • Whole genome sequencing to assess off-target effects.
  • Generation of transgenic plants for inducible and tissue-specific gene editing.

Main Results:

  • AcrVA1 demonstrated robust inhibition of Mb2Cas12a and LbCas12a in vitro.
  • AcrVA1 effectively inhibited LbCas12a-mediated genome editing in rice protoplasts and transgenic lines.
  • Co-expression of AcrVA1 significantly reduced off-target mutations induced by CRISPR-LbCas12a.
  • Controlling AcrVA1 expression enabled inducible, tissue-specific genome editing and synthetic logic gates for gene regulation.

Conclusions:

  • An efficient anti-CRISPR-Cas12a system has been established for plant applications.
  • This system offers precise control over genome editing, mitigating off-target effects and enabling fine-tuning of editing efficiency.
  • The developed system facilitates spatial-temporal control of genome editing and the creation of synthetic gene circuits in plants.