Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2025

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

10.9K

Evolving dual-trait EPSP synthase variants using a synthetic yeast selection system.

Kevin B Reed1, Wantae Kim1, Hongyuan Lu1

  • 1McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712.

Proceedings of the National Academy of Sciences of the United States of America
|August 19, 2024
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent advances in antiviral drugs for Chikungunya virus (CHIKV): Targets, mechanisms, and development strategies.

Acta pharmaceutica Sinica. B·2026
Same author

Engineering Biosensors to Enhance Monoterpene Indole Alkaloid Production in Yeast.

bioRxiv : the preprint server for biology·2026
Same author

A tunable CTD grammar governs the spatial programming of the transcription cycle.

Nature communications·2026
Same author

A Linear Mixed Effects Model for Evaluating Synthetic Gene Circuits.

ACS synthetic biology·2026
Same author

Comparison of the clinical efficacy, safety and EEG functional connectivity changes between 18-Hz rTMS and iTBS of accelerated dTMS treatment for major depressive disorder: a randomized controlled trial.

Molecular psychiatry·2026
Same author

DKK1 Suppresses Hippo Signaling via PIP<sub>3</sub>-OGT-LRP6 <i>O</i>-GlcNAcylation in Hepatocellular Carcinoma.

Cancer communications (London, England)·2026
This summary is machine-generated.

Researchers developed a synthetic yeast system to evolve 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) for enhanced glyphosate tolerance and catalytic efficiency. A single round of evolution yielded a mutant with robust herbicide resistance and improved enzyme function.

Area of Science:

  • Biochemistry
  • Plant Science
  • Synthetic Biology

Background:

  • 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is crucial for aromatic amino acid synthesis in plants and is the target of glyphosate herbicide.
  • Existing glyphosate-resistant EPSPS variants often exhibit reduced catalytic efficiency, necessitating new variants with dual-trait improvements.

Purpose of the Study:

  • To establish a synthetic yeast system for rapid evolution of EPSPS with both high glyphosate tolerance and catalytic efficiency.
  • To identify novel EPSPS mutants that overcome the typical trade-off between herbicide resistance and enzyme performance.

Main Methods:

  • A synthetic yeast system was created to study and evolve heterologous EPSP synthases.
  • The system was validated using known EPSPS variants to confirm recapitulation of plant growth characteristics under glyphosate stress.
Keywords:
EPSPSdirected evolutionglyphosatesynthetic biologyyeast

More Related Videos

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

1.2K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.5K

Related Experiment Videos

Last Updated: Jun 16, 2025

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

10.9K
Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

1.2K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.5K
  • Mutagenesis libraries were screened under strong dual-trait selection pressure.

    • Main Results:

    • The synthetic yeast system successfully recapitulated plant growth responses to glyphosate.
    • A single round of evolution under dual-trait selection pressure identified a superior EPSPS mutant.
    • The evolved mutant demonstrated robust glyphosate tolerance (Ki ~1 mM) and a ~2.5-fold increase in enzymatic efficiency compared to the starting enzyme.
    • Crystal structures revealed insights into the mutations conferring improved dual traits.

    Conclusions:

    • The developed synthetic yeast platform is effective for evolving EPSPS with improved glyphosate resistance and catalytic efficiency.
    • Novel EPSPS mutants were identified with the potential to outperform existing resistant variants in whole-plant applications.
    • This platform facilitates the exploration of the complex relationship between glyphosate resistance and enzymatic efficiency in EPSPS.