Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

2.4K
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
2.4K
Bone Formation by Intramembranous Ossification01:29

Bone Formation by Intramembranous Ossification

5.8K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
5.8K
Fibril-associated Collagen01:11

Fibril-associated Collagen

2.5K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.5K
Structural Protein Function01:56

Structural Protein Function

27.5K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
27.5K
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

4.1K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
4.1K
Fibrous Proteins00:55

Fibrous Proteins

2.1K
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inositol Thiophosphates as Inhibitors of Mammalian, Plant, and Fungal Phytases.

ACS chemical biology·2026
Same author

Fibrillin-1 Inhibits Early Adipogenic Commitment Via αvβ3 Integrin Signaling.

Journal of molecular biology·2026
Same author

Corrigendum to "Tissue material properties, whole-bone morphology and mechanical behavior in the <i>Fbn1</i> <sup>C1041G/+</sup> mouse model of Marfan Syndrome" [Matrix Biol. Plus 23 (2024) 100155].

Matrix biology plus·2025
Same author

Fluorescent mapping of osteocyte-driven bone formation at pre-osteocyte and mature osteocyte lacunae.

Acta biomaterialia·2025
Same author

Double-Edged Signaling: FAK's Role in Aortic Dissection Progression.

JACC. Basic to translational science·2025
Same author

Exploring thoracic aorta ECM alterations in Marfan syndrome: insights into aorta wall structure.

Scientific reports·2025
Same journal

Airway macrophage specific glycocalyx expression and remodeling following viral infection.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Cartilage intermediate layer protein (CILP) in cardiac fibrosis: Protective or pathogenic?

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Polymerizing laminins: Assembly, functions and disorders.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Oligodendrocyte integrin-β1 regulates blood-brain barrier and remyelination in hemorrhagic brain.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Dynamic regulation of the tissue microenvironment by integrins and the extracellular matrix.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Corrigendum to "Basement membrane components define the microenvironment of aggregated fibroblasts in the skin and support their aggregation in vitro" [Matrix Biology 146 (2026) 102008].

Matrix biology : journal of the International Society for Matrix Biology·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2025

Culturing and Measuring Fetal and Newborn Murine Long Bones
06:58

Culturing and Measuring Fetal and Newborn Murine Long Bones

Published on: April 26, 2019

8.2K

Fibronectin isoforms promote postnatal skeletal development.

Neha E H Dinesh1, Nissan Baratang2, Justine Rosseau2

  • 1Faculty of Medicine and Health Sciences, Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada.

Matrix Biology : Journal of the International Society for Matrix Biology
|August 19, 2024
PubMed
Summary
This summary is machine-generated.

Fibronectin (FN) is vital for skeletal development. Both cellular (cFN) and plasma (pFN) isoforms are crucial for chondrogenesis, cartilage maturation, and bone formation, impacting overall growth.

Keywords:
ChondrocytesChondrogenesisDifferentiationFibronectinGrowth PlateSkeletal development

More Related Videos

Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors FAPs and Myogenic Progenitors MPs in Skeletal Muscle in the Rat
09:49

Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors FAPs and Myogenic Progenitors MPs in Skeletal Muscle in the Rat

Published on: June 9, 2021

5.9K
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.0K

Related Experiment Videos

Last Updated: Jun 16, 2025

Culturing and Measuring Fetal and Newborn Murine Long Bones
06:58

Culturing and Measuring Fetal and Newborn Murine Long Bones

Published on: April 26, 2019

8.2K
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors FAPs and Myogenic Progenitors MPs in Skeletal Muscle in the Rat
09:49

Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors FAPs and Myogenic Progenitors MPs in Skeletal Muscle in the Rat

Published on: June 9, 2021

5.9K
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.0K

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Orthopedics

Background:

  • Fibronectin (FN) is an extracellular matrix glycoprotein critical for tissue development.
  • Mutations in FN are linked to spondylometaphyseal dysplasia, highlighting its role in skeletal health.
  • The specific roles of FN isoforms in skeletal development remain largely uncharacterized.

Purpose of the Study:

  • To investigate the functional importance of cellular FN (cFN) and plasma FN (pFN) isoforms in skeletal development.
  • To elucidate the distinct and combined roles of cFN and pFN in chondrogenesis and bone formation.
  • To identify the molecular pathways regulated by FN during skeletal growth.

Main Methods:

  • Generation of conditional knockout mouse models: cFNKO (cartilage-specific), pFNKO (hepatocyte-specific), and FNdKO (double knockout).
  • Analysis of skeletal parameters from postnatal day one to adulthood (two months).
  • Micro-CT scanning for bone microarchitecture, histological analysis of growth plates, and assessment of chondrogenic and bone formation markers.

Main Results:

  • Distinct topological deposition patterns of FN isoforms were observed during skeletal development.
  • Deletion of individual FN isoforms had subtle effects, but the double knockout (FNdKO) significantly reduced body and bone length.
  • FNdKO mice exhibited impaired bone microarchitecture, reduced bone mineral density, increased bone marrow adiposity, altered chondrogenesis, and dysregulated TGF-β1/AKT signaling.

Conclusions:

  • Fibronectin is essential for chondrogenesis, cartilage maturation, and trabecular bone formation.
  • Both cFN and pFN isoforms play critical, albeit regionally distinct, roles in regulating skeletal development.
  • FN-mediated signaling pathways are crucial for overall skeletal growth and homeostasis.