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Cell sorting of lymphocyte subpopulations by biotinylated antibody and avidin-Sepharose beads

R E Cone, T Chi

    Journal of Experimental Pathology
    |January 1, 1985
    PubMed

    A method has been developed to divide lymphocyte populations into subsets based on the affinity of avidin for biotinylated antibodies. Murine splenic lymphocytes were mixed with biotin-conjugated anti-immunoglobulin, Thy-1 or anti-Lyt antibodies. Cells bound by these reagents were adsorbed to avidin-sepharose beads and by this negative selection procedure effluent cell populations enriched for T cells, B cells or Lyt subsets were prepared reproducibly and rapidly.

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