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Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
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Screening Preeclampsia Genes and the Effects of CITED2 on Trophoblastic Function

Xiujing Lu1, Xi Lan1, Xiaoqian Fu1

  • 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, People's Republic of China.

International Journal of General Medicine
|August 20, 2024
PubMed

Insights

CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl-terminal domain 2 (CITED2) is upregulated in preeclampsia (PE) placentas and affects trophoblast function, suggesting it as a potential therapeutic target for PE.

Area of Science:

  • Obstetrics and Gynecology
  • Molecular Biology
  • Genetics

Background:

  • Preeclampsia (PE) is a significant obstetric complication with unclear mechanisms.
  • Dysregulation of CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl-terminal domain 2 (CITED2) is implicated in various disorders.
  • The specific role of CITED2 in PE pathogenesis remains largely unknown.

Purpose of the Study:

  • To identify hub genes associated with preeclampsia.
  • To investigate the role of CITED2 in trophoblast cells using bioinformatic and experimental approaches.

Main Methods:

  • Bioinformatic analysis of Gene Expression Omnibus (GEO) datasets to identify PE-related hub genes.
  • Immune infiltration and enrichment analyses to determine related pathways and immune cells.
  • Validation of CITED2 mRNA and protein levels in PE placentas via PCR and Western Blot (WB).
  • In vitro functional assays using siRNA to knockdown CITED2 in trophoblast cells.

Main Results:

  • Six PE-related genes were enriched in NOTCH signaling pathways, glycolysis, and hypoxia.
  • Immune infiltration analysis indicated a role for activated NK cells and regulatory T cells.
  • CITED2 expression was significantly upregulated in PE placentas.
  • Knockdown of CITED2 impaired trophoblast cell migration, invasion, and proliferation, while enhancing apoptosis.

Conclusions:

  • CITED2 plays a crucial role in regulating trophoblast cell function.
  • CITED2 is a potential therapeutic target for managing preeclampsia.
Abstract

Keywords:
CITED2bioinformaticscell functionplacentapreeclampsia

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