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Related Concept Videos

CRISPR01:59

CRISPR

50.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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Related Experiment Video

Updated: Jun 16, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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A CRISPR-dCas13 RNA-editing tool to study alternative splicing.

Yaiza Núñez-Álvarez1, Tristan Espie-Caullet1,2,3, Géraldine Buhagiar2,3

  • 1Institut de Génétique Humaine, Université de Montpellier, CNRS UMR9002, Montpellier, France.

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Summary

This study introduces dCasRx, a novel CRISPR tool for precisely altering alternative splicing patterns. This technology helps researchers understand splicing

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Alternative splicing generates diverse proteins from a single gene, impacting biological processes and diseases.
  • The precise roles of splicing isoforms and their regulatory elements are challenging to study.
  • Limitations in current methods hinder the investigation of alternative splicing's significance.

Purpose of the Study:

  • To develop a cost-effective method for switching alternative splicing patterns of endogenous transcripts.
  • To identify key regulatory RNA elements controlling specific splicing events.
  • To expand the RNA toolkit for understanding alternative splicing mechanisms and physiological impacts.

Main Methods:

  • Utilized dCasRx, a catalytically inactive RNA-targeting CRISPR-dCas13 ortholog.
  • Applied the dCasRx system to efficiently switch alternative splicing patterns.
  • Demonstrated a new application for identifying regulatory RNA elements.

Main Results:

  • Successfully switched alternative splicing patterns of endogenous transcripts using dCasRx.
  • Achieved this without altering overall gene expression levels.
  • Validated the dCasRx system's utility in identifying key regulatory RNA elements for splicing events.

Conclusions:

  • dCasRx offers a powerful and cost-effective approach to manipulate alternative splicing.
  • This splice-editing system facilitates the identification of critical RNA regulatory elements.
  • The expanded RNA toolkit enhances the understanding of alternative splicing in health and disease.