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Updated: Jun 16, 2025
Fluorescence in situ hybridization FISH Protocol in Human Sperm
Published on: September 1, 2009
Assessment of sperm chromosomal abnormalities using fluorescence in situ hybridization (FISH): implications for
Francesca Paola Luongo1, Eugenia Annunzi1, Francesca Girolamo1
1Department of Molecular Medicine and Development, University of Siena, 53100, Siena, Italy.
Purpose:
Chromosomal abnormalities play an important role in male infertility, which is becoming a significant issue in human fertility. Aim of this study was to evaluate the incidence of spermatic aneuploidies and diploidies in human sperm, according to semen parameters.
Methods:
We performed semen analysis according to the 6th edition of WHO criteria in 50 male subjects; samples were divided into normozoospermic (n = 23) or those with altered seminal parameters (n = 27). To assess chromosomal numerical alterations of sperm, fluorescence in situ hybridization (FISH) was used.
Result:
A significant increase in aneuploidies and diploidies was observed in samples with altered seminal parameters. Furthermore, stratifying this group, we observed a significant increase in aneuploidies and total abnormalities in oligozoospermic, asthenoteratozoospermic (AT), and oligoteratoasthenozoospermic (OAT) samples compared to normozoospermic.
Conclusion:
Our results showed the correlation between altered seminal parameters and numerical chromosomal abnormalities, confirming that sperm FISH analysis could be an additional clinical tool to assess reproductive potential in infertile males. Moreover, our results point to the importance of updating the normality ranges for detecting chromosomal aneuploidies using FISH.
