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Related Experiment Video

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Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure
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Ethanol-related transcriptomic changes in mouse testes.

Gwidong Han1, Seung Jae Lee1, Seung Pyo Hong1

  • 1School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea.

BMC Genomics
|August 20, 2024
PubMed
Summary

Chronic ethanol consumption in mice reduced sperm count and motility. This study reveals down-regulated gene expression in sperm tail structures, suggesting a molecular basis for alcohol-induced male reproductive dysfunction.

Keywords:
AlcoholEthanolSperm motilitySpermatogenesisTestis

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Area of Science:

  • Reproductive biology
  • Toxicology
  • Molecular biology

Background:

  • Alcohol consumption negatively impacts multiple organs.
  • Previous research focused on physiological and cellular effects of ethanol on male reproduction.
  • No systematic studies investigated ethanol's impact on male reproductive gene expression.

Purpose of the Study:

  • To conduct a comprehensive transcriptomic analysis of ethanol-induced changes in the mouse testis.
  • To identify specific genes and pathways affected by chronic ethanol administration.
  • To explore the molecular mechanisms underlying ethanol-mediated male reproductive dysfunction.

Main Methods:

  • Chronic ethanol administration using the Lieber-DeCarli diet in mice.
  • Assessment of testicular and epididymal integrity, sperm morphology, count, and motility.
  • Total RNA sequencing of testicular tissue.
  • In silico analyses, including gene set enrichment analysis.

Main Results:

  • Ethanol-fed mice exhibited normal testicular histology and sperm morphology but reduced sperm count and motility.
  • RNA sequencing revealed differential expression in approximately 2% of testicular genes, with 28% being cell-type specific.
  • Gene set enrichment analysis identified down-regulation of sperm tail structure-related genes, including Forkhead box J1 (Foxj1).

Conclusions:

  • This study presents the first comprehensive transcriptomic profile of ethanol's effects on the mouse testis.
  • Altered gene expression in the testis is a potential mechanism for ethanol-induced male reproductive dysfunction.
  • Impaired sperm motility may result from ethanol-induced changes in the expression of genes related to sperm structure and function.