Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

816
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
816
Nitrosation of Enols01:19

Nitrosation of Enols

2.6K
The nitrosation reaction is one of the methods of preparing 1,2-diketones. The enol tautomer of the starting ketone reacts with sodium nitrite in hydrochloric acid, generating the 1,2-diketone after hydrolysis.
2.6K
2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

4.1K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
4.1K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

948
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
948
Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

646
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
646
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview01:26

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview

3.3K
Nitrous acid and nitric acids are two types of acids containing nitrogen, among which nitrous acid is weaker than nitric acid. Nitrous acid with a pKa value of 3.37 ionizes in water to give a nitrite ion and the hydronium ion.
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrafast Photochemical Reaction Dynamics of 3-Phenyl-1,4,2-dioxazol-5-one Revealed by Femtosecond Time-Resolved Infrared Spectroscopy.

International journal of molecular sciences·2026
Same author

Temperature-Dependent Surface Structural Change in Self-Assembled Monolayers Studied with Vibrational Sum-Frequency Generation and QM/MD Simulation.

The journal of physical chemistry letters·2025
Same author

Linker-Engineered Tyrosine-Azide Coatings for Stable Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC) Functionalization.

Polymers·2025
Same author

Approximate Spin-Orbit Eigenstates with Static and Dynamical Correlations: X2CSO-DSRG-MRPT2 Prescription.

Journal of chemical theory and computation·2025
Same author

Deciphering Charge Transfer and Hydrogen Bonding Characteristics from Liquid Water XAS Spectra.

Journal of chemical theory and computation·2025
Same author

Ultrafast Photochemical Dynamics of Dinitrosyl Iron Complexes Investigated by Femtosecond Time-Resolved Infrared Spectroscopy.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jun 15, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

3.8K

Dynamics of NO Release and Linkage Isomer Formation from S-Nitroso-Mercaptoethanol in Aqueous Solutions: Insights

Hojeong Yoon1, Seongchul Park1,2, So Yeon Kim3

  • 1Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Korea.

The Journal of Physical Chemistry Letters
|August 21, 2024
PubMed
Summary

Researchers studied how S-nitroso-thiol (RSNO) molecules break apart and reform after light exposure. They discovered a new sulfur-ON linkage isomer and its rapid isomerization back to the original RSNO in water.

More Related Videos

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

19.0K
Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.0K

Related Experiment Videos

Last Updated: Jun 15, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

3.8K
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

19.0K
Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.0K

Area of Science:

  • Photochemistry
  • Chemical Dynamics
  • Spectroscopy

Background:

  • S-nitroso-thiols (RSNOs) are crucial biological NO transporters.
  • Understanding RSNO photodynamics is key for photochemical applications.

Purpose of the Study:

  • Investigate the photoexcitation dynamics of S-nitroso-mercaptoethanol (MceSNO) in aqueous solution.
  • Characterize NO dissociation, recombination, and linkage isomerization pathways.

Main Methods:

  • Femtosecond infrared spectroscopy
  • High-level quantum calculations (CASPT2D(16,11)/cc-pVTZ)
  • Potential energy surface construction

Main Results:

  • 320 nm excitation of MceSNO leads to rapid NO and MceS radical dissociation.
  • MceS radicals form MceSSMce (31%) or recombine with NO (70%).
  • A novel sulfur-ON linkage isomer (MceSON) forms (28%) and isomerizes back to MceSNO in ~470 ps.

Conclusions:

  • The study elucidates the complex photodynamics of MceSNO in water.
  • A new MceSON isomer and its isomerization pathway were identified.
  • Low isomerization barrier (3.3 kcal/mol) suggests facile interconversion.