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Perovskites as Multiphoton Fluorescence Contrast Agents for In Vivo Imaging
Peuli Nath1, Adrian Ross Liversage2, Luke J Mortensen2,3
1Department of Physics & Astronomy, University of Toledo, Toledo, Ohio 43606, United States.
ACS Applied Materials & Interfaces
|August 21, 2024
Summary
Perovskite nanocrystals (PNCs) show promise for deep tissue imaging due to their strong multiphoton absorption and high fluorescence. These novel contrast agents enable visualization of cells in living mice, advancing biological research.
Area of Science:
- Biomedical Optics
- Materials Science
- Nanotechnology
Background:
- Multiphoton fluorescence microscopy requires materials with high multiphoton absorption cross-sections and quantum yields for optimal deep tissue imaging.
- Perovskite nanocrystals (PNCs) possess exceptionally large multiphoton absorption cross-sections (up to five-photon) and near-unity fluorescence quantum yields.
Purpose of the Study:
- To evaluate PNCs as contrast agents for multiphoton imaging of cells in living organisms.
- To explore the potential of PNCs for deep tissue imaging applications.
Main Methods:
- Perovskite nanocrystals (CsPbX3, PNCs) were synthesized and stabilized within a SiO2 matrix (60-70 nm diameter).
- In vivo multiphoton fluorescence microscopy was performed using PNCs to image mesenchymal stromal cells in a living mouse.
Main Results:
- PNCs demonstrated strong multiphoton absorption and high fluorescence quantum yield, suitable for deep tissue imaging.
- Mesenchymal stromal cells in a living mouse were successfully imaged using PNCs as contrast agents.
- The SiO2 encapsulation provides a platform for surface modification for targeted biological applications.
Conclusions:
- PNCs are highly effective contrast agents for multiphoton microscopy in biological tissues.
- This technology offers significant benefits for dynamic cell tracking in deep tissues, aiding in understanding cellular processes in health and disease.
