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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

14.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Related Experiment Video

Updated: May 5, 2026

Swab Sampling Method for the Detection of Human Norovirus on Surfaces
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Swab Sampling Method for the Detection of Human Norovirus on Surfaces

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CRISPR/Cas13a analysis based on NASBA amplification for norovirus detection.

Zefeng Mao1, Huang Lei2, Ruipeng Chen3

  • 1School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China; Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China.

Talanta
|August 21, 2024
PubMed
Summary

A new NASBA/Cas13a method rapidly detects human norovirus (HuNoV) in food. This sensitive assay simplifies detection by combining amplification and CRISPR recognition, offering accurate results comparable to qRT-PCR.

Keywords:
BiosensingCRISPR/Cas13aNucleic acid testingRNA amplification

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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Area of Science:

  • Molecular biology
  • Biotechnology
  • Food safety

Background:

  • Human norovirus (HuNoV) is a major cause of foodborne illness globally.
  • Rapid and accurate detection of HuNoV is essential for public health.
  • Existing CRISPR/Cas13a assays can be complex due to external transcription steps.

Purpose of the Study:

  • To develop an efficient and simplified diagnostic technique for human norovirus detection.
  • To integrate Nucleic Acid Sequence-Based Amplification (NASBA) with the CRISPR/Cas13a system.
  • To establish a rapid, sensitive, and accurate method for identifying HuNoV.

Main Methods:

  • Developed a NASBA/Cas13a system targeting conserved regions of the norovirus genome.
  • Optimized reaction kinetics for Cas13a and the cascade reaction.
  • Validated the method by detecting norovirus in shellfish samples.

Main Results:

  • Achieved a low detection limit of 51 picomolar (pM) for Cas13a.
  • The NASBA/Cas13a method demonstrated high sensitivity, detecting viral loads as low as 10 copies/μL.
  • Results from shellfish samples were comparable to quantitative reverse transcription PCR (qRT-PCR) but in less time.

Conclusions:

  • The NASBA/Cas13a system provides a sensitive, accurate, and rapid diagnostic tool for human norovirus.
  • This integrated approach simplifies detection by eliminating external transcription steps.
  • The method shows significant potential for food safety applications and public health surveillance.