Related Experiment Video
Updated: Jun 15, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Cotton HD-Zip I transcription factor GhHB4-like regulates the plant response to salt stress
Aiping Ren1, Tianyang Wen1, Xiao Xu1
1Zhengzhou Research Base, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Abstract:
Soil salinity is a major environmental constraint to plant production. The homeodomain-leucine zipper I (HD-Zip I) transcription factors play a crucial role in growth, development and defence responses of plants. However, the function and underlying mechanism of HD-Zip I in cotton remain unexplored. This study investigated the role of GhHB4-like, a cotton HD-Zip I gene, in plant tolerance to salt stress. Ectopic expression of GhHB4-like gene enhanced, while its silencing impaired the salt tolerance in Arabidopsis. Y1H and effector-reporter assays revealed that GhHB4-like activated the expression of GhNAC007, which is essential for salt resistance. Knock-down of GhNAC007 also impaired salt resistance of cotton plants. In addition, GhHB4-like-GhNAC007 might have positively regulated the expression of GhMYB96 and ABA signalling-related genes, thereby leading to enhanced salt resistance. Interestingly, deleting motifs 3 and 5 near the 3'-end of GhHB4-like significantly enhanced GhNAC007 activation, indicating that both motifs acted as transcriptional activation inhibitory domains. The results suggest that GhHB4-like-GhNAC007 regulated plant response to salt stress, potentially by modulating GhMYB96 and ABA signalling-related genes.
More Related Videos
Related Concept Videos
Responses to Salt Stress
Responses to Heat and Cold Stress
Cell Signaling in Plants
Regulation of Transpiration by Stomata
Responses to Drought and Flooding
Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

