Phage Immunoprecipitation and Sequencing-a Versatile Technique for Mapping the Antibody Reactome.

Area of Science:

• Immunology
• Biotechnology
• Genomics

Background:

• Circulating antibodies reveal insights into pathogen exposure, allergies, and autoimmune diseases.
• Understanding the antibody reactome is crucial for biomarker discovery, diagnosis, and prognosis.
• Phage display immunoprecipitation and sequencing (PhIP-seq) is an emerging high-throughput technology.

Purpose of the Study:

• To comprehensively review the history and recent advances in PhIP-seq.
• To discuss improvements in PhIP-seq library design and diverse applications.

Main Methods:

• PhIP-seq utilizes bioinformatically designed phage display libraries with overlapping protein segments.
• Antibody immunoprecipitation is performed using these libraries with circulating antibodies.
• Next-generation sequencing of barcoded DNA inserts from bound phages enables hit determination.

Main Results:

• PhIP-seq has rapidly advanced as a powerful technique for antibody reactome characterization.
• Recent developments have enhanced library design and expanded PhIP-seq applications.
• The method allows for parallel sample analysis from multiple sources.

Conclusions:

• PhIP-seq is a valuable tool for dissecting the antibody reactome.
• Continued advancements promise broader applications in immunological research and diagnostics.
• This review provides a comprehensive overview for researchers in the field.