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The Estrogen Receptor-Related Orphan Receptors Regulate Autophagy through TFEB.

McKenna Losby1, Matthew Hayes1, Aurore Valfort1

  • 1Division of Biology & Biomedical Sciences, Washington University School of Medicine, St. Louis (M.L.); Department of Pharmacodynamics, University of Florida College of Pharmacy, Gainesville, Florida (M.H., A.V., R.S., T.P.B.); University of Florida Genetics Institute, Gainesville, Florida (T.P.B.); Brown Foundation Institute of Molecular Medicine, McGovern Medical School, UTHealth, Houston, Texas, (D.H.S., V.A.N.); Department of Pharmacology and Physiology, Saint Louis University School of Medicine, St. Louis, Missouri (J.K.W.); Department of Molecular and Human Genetics, Baylor College of Medicine, Houston TX (W.X., L.Z.); and Center for Clinical Pharmacology, St Louis College of Pharmacy, University of Health Sciences and Pharmacy, St. Louis MO (C.B.).

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Estrogen receptor-related receptors (ERRs) activate the autophagy pathway by increasing Transcription factor EB (TFEB) expression. This mechanism offers a potential therapeutic strategy for heart failure and other diseases.

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Area of Science:

  • Cardiovascular Biology
  • Cellular Metabolism
  • Molecular Endocrinology

Background:

  • Autophagy is a vital cellular process for maintaining homeostasis.
  • Estrogen receptor-related receptors (ERRs) regulate cardiac metabolism and function.
  • Previous studies indicated ERR agonists improve cardiac function and induce autophagy.

Purpose of the Study:

  • To elucidate the mechanism by which ERRs induce autophagy in cardiomyocytes.
  • To investigate the role of Transcription factor EB (TFEB) in ERR-mediated autophagy.

Main Methods:

  • Utilized neonatal rat ventricular myocytes and C2C12 myoblasts.
  • Investigated the direct transcriptional regulation of TFEB by ERRs.
  • Assessed the impact of ERR activation on TFEB and downstream autophagy-related gene expression.

Main Results:

  • Identified TFEB as a direct target gene of ERRs.
  • Demonstrated that ERR agonists increase TFEB expression in cardiomyocytes and myoblasts.
  • Showed that increased TFEB leads to enhanced expression of autophagy-stimulating genes.

Conclusions:

  • ERR activation directly induces TFEB, a master regulator of autophagy.
  • This ERR-TFEB-autophagy axis represents a novel therapeutic pathway.
  • Targeting ERRs may offer a promising treatment for heart failure and other autophagy-related diseases.