Related Experiment Video
Updated: Jun 15, 2025

Gene Expression Analyses in Human Follicles
Published on: February 17, 2023
In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular
Ilmatar Rooda1,2,3, Jasmin Hassan4,5, Jie Hao4,5,6
1Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden. ilmatar.rooda@ki.se.
Abstract:
The ovarian cortical reserve of follicles is vital for fertility. Some medical treatments are toxic to follicles, leading to premature ovarian insufficiency. Ovarian tissue cryopreservation is an established method to preserve fertility in adults and even applied in prepuberty despite unproven efficacy. Here, we analyze transcriptomes of 120 cortical follicles from children and adults for detailed comparison. We discover heterogeneity with two main types of follicles in both age groups: one with expected oocyte-granulosa profiles and another with predicted role in signaling. Transcriptional changes during growth to the secondary stage are similar overall in children and adults, but variations related to extracellular matrix, theca cells, and miRNA profiles are found. Notably, cyclophosphamide dose correlates with interferon signaling in child follicles. Additionally, morphology alone is insufficient for follicle categorization suggesting a need for additional markers. Marker genes for early follicle activation are determined. These findings will help refine follicular classification and fertility preservation techniques across critical ages.
More Related Videos
09:11Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
Published on: September 27, 2022
06:36Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles
Published on: November 30, 2022
Related Concept Videos
Folliculogenesis
Oogenesis
Ovarian Cycle