Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-Modality Alignment of Spatial Transcriptomics, Multiplexed Imaging, and Histology with PHARAOH.

Research square·2026
Same author

A pilot translational study of neoadjuvant fulvestrant plus abemaciclib in women with advanced low-grade serous carcinoma.

Nature communications·2026
Same author

Cross-cultural adaptation, validity, and reliability study of the Chinese version of the vestibular activities avoidance Instrument-9.

Journal of vestibular research : equilibrium & orientation·2026
Same author

Tumor microbial burden drives immune responses through regulation of Interferon signaling.

Cancer discovery·2026
Same author

Molecular phenotypes and spatial archetypes: A new framework for cancer-associated fibroblasts.

Cancer cell·2026
Same author

MHC Attention: Identifying HLA-E presented cancer antigens through deep learning and high-throughput screening.

bioRxiv : the preprint server for biology·2026
Same journal

Human aminoacyl-tRNA synthetases as integrators of translation and cell signalling networks.

Nature reviews. Molecular cell biology·2026
Same journal

How proteins fold.

Nature reviews. Molecular cell biology·2026
Same journal

Single-cell evidence for PANoptosome complexes.

Nature reviews. Molecular cell biology·2026
Same journal

Reply to 'Single-cell evidence for PANoptosome complexes'.

Nature reviews. Molecular cell biology·2026
Same journal

Plucking cellular ribosomes with Ribo-Tweezer.

Nature reviews. Molecular cell biology·2026
Same journal

COPII meets autophagy at the ER membrane.

Nature reviews. Molecular cell biology·2026
See all related articles

Related Experiment Video

Updated: Jun 15, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K

Profiling cell identity and tissue architecture with single-cell and spatial transcriptomics.

Gunsagar S Gulati1, Jeremy Philip D'Silva2, Yunhe Liu3

  • 1Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.

Nature Reviews. Molecular Cell Biology
|August 21, 2024
PubMed
Summary
This summary is machine-generated.

Single-cell and spatial transcriptomics reveal cellular diversity and tissue microenvironments. These powerful tools are advancing biological research and clinical translation through innovative computational methods.

More Related Videos

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K

Related Experiment Videos

Last Updated: Jun 15, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Single-cell transcriptomics has significantly advanced the understanding of cellular heterogeneity in health and disease.
  • Spatial transcriptomics provides crucial context by mapping cells within their multicellular neighborhoods, identifying tissue ecotypes.

Purpose of the Study:

  • To review recent advancements in single-cell and spatial transcriptomics technologies and computational methodologies.
  • To discuss challenges and future prospects in analyzing complex transcriptomic data for biological discovery and clinical applications.

Main Methods:

  • Analysis of large-scale datasets from targeted and whole-transcriptome profiling of millions of cells.
  • Integration of sample processing, data integration, cell state identification, trajectory modeling, deconvolution, and spatial analysis techniques.
  • Application of deep learning and foundation models for advanced transcriptomic data analysis.

Main Results:

  • New insights into developmental hierarchies, cellular plasticity, and tissue microenvironments.
  • Identification of spatially recurrent cellular phenotypes (ecotypes) and subtle cell states.
  • Demonstrated utility of advanced computational methods, including deep learning, for transcriptomic data interpretation.

Conclusions:

  • Single-cell and spatial transcriptomics are revolutionizing biological research by providing unprecedented cellular and spatial resolution.
  • These technologies hold significant promise for translation to clinical applications in fields such as stem cell biology, immunology, and oncology.