Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-state diagram for DNA.

V I Ivanov1, Krylov DYu, E E Minyat

  • 1Institute of Molecular Biology, USSR Academy of Sciences, Moscow.

Journal of Biomolecular Structure & Dynamics
|August 1, 1985
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Results of modified submandibular surgical approach usage for the mouth floor phlegmons treatment with respect to dynamic of local non-specific resistance].

Stomatologiia·2022
Same author

Taxonomic diversity of sputum microbiome in lung cancer patients and its relationship with chromosomal aberrations in blood lymphocytes.

Scientific reports·2020
Same author

[Vitamin D and laboratory indicators of cardiovascular risk in the elderly.]

Klinicheskaia laboratornaia diagnostika·2020
Same author

[Biophysical aspects of biological activity structure--strain calcium carbonat in micellar form].

Gigiena i sanitariia·2014
Same author

[Effect of antibiotics on antigenic properties of Vibrio comma].

Antibiotiki·2014
Same author

[Hemoperitoneum as a predictor of the traumatic process complications in the injured persons with severe combined trauma].

Klinichna khirurhiia·2013
Same journal

Decrypting the allosteric network: multi-scale dynamics of SARS-CoV-2 main protease inhibition by <i>Moringa oleifera</i> flavonoids.

Journal of biomolecular structure & dynamics·2026
Same journal

Anti-mycobacterial activity of phytocompounds from <i>Ricinus communis</i> L. - an integrated <i>in-vitro</i> and <i>in-silico</i> approach.

Journal of biomolecular structure & dynamics·2026
Same journal

Binding studies of the X-ray characterized [SnMe<sub>2</sub>Cl<sub>2</sub>(Me<sub>2</sub>phen)] complex with human serum albumin: experimental and molecular docking approaches.

Journal of biomolecular structure & dynamics·2026
Same journal

Computational design and experimental validation of peptide inhibitors to disrupt urease enzyme maturation in pathogenic bacteria <i>Proteus mirabilis</i>.

Journal of biomolecular structure & dynamics·2026
Same journal

Wavelet-domain multiway spectral separation of free drug, DNA, and drug-DNA complex profiles for quantitative binding analysis based on fractional occupancy (<i>θ</i>).

Journal of biomolecular structure & dynamics·2026
Same journal

Gene expression and microsecond scale conformational dynamics suggest potential regulatory mechanisms for the expanded subtilase family of <i>T. rubrum</i>.

Journal of biomolecular structure & dynamics·2026
See all related articles

This study maps DNA phase diagrams, revealing DNA cooperativity lengths and energies. New effects observed include increased melting temperature and wider melting curves due to multiple DNA conformations coexisting.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Polymer Science

Background:

  • DNA exists in various forms, including A, B, and Coil.
  • Understanding DNA conformational transitions is crucial for molecular biology.
  • Poly(dA-dT) is a model system for studying DNA structure and transitions.

Purpose of the Study:

  • To construct experimental phase diagrams for natural DNAs and poly(dA-dT).
  • To determine the cooperativity length (nu o) of the B-A DNA transition.
  • To investigate the effects of coexisting DNA conformations on phase behavior.

Main Methods:

  • Experimental phase diagram construction in coordinates of alcohol fraction and temperature.
  • Estimation of cooperativity length (nu o) from the slopes of phase transition branches.

Related Experiment Videos

  • Analysis of DNA melting curves near the triple point.

    • Main Results:

    • Phase diagrams revealed A, B, and Coil forms of DNA.
    • The cooperativity length (nu o) for the B-A transition was estimated at 10-20 base pairs.
    • An energy of 1.2-1.8 kcal/mol was calculated for the B/A DNA junction.
    • Two novel effects were observed: increased melting temperature and a widened melting curve due to the coexistence of three conformations.

    Conclusions:

    • The coexistence of multiple DNA conformations influences DNA phase behavior.
    • The findings provide insights into DNA structural transitions and stability.
    • This research contributes to understanding DNA polymorphism and its implications.