Optimizing Polyhydroxyalkanoate production using a novel Bacillus paranthracis isolate: A response surface...

Area of Science:

• Microbiology and Biotechnology
• Polymer Science
• Environmental Science

Background:

• Microorganisms offer sustainable routes for bioproducts like Polyhydroxyalkanoate (PHA).
• PHA is a biodegradable polymer with potential to replace petroleum-based plastics.
• Utilizing waste streams like sewage for bioproducts enhances sustainability.

Purpose of the Study:

• To screen and identify a novel bacterial strain for Polyhydroxyalkanoate (PHA) production from sewage.
• To optimize PHA production using statistical experimental design.
• To evaluate the potential of the identified strain for eco-friendly biopolymer manufacturing.

Main Methods:

• Screening of a novel isolate, Bacillus paranthracis RSKS-3, for PHA production.
• Whole Genome Sequencing for strain identification.
• Plackett-Burman design and Response Surface Methodology (RSM) using Design Expert 12.0 for optimization.

Main Results:

• Bacillus paranthracis RSKS-3 was identified as a PHA producer from sewage.
• Key factors affecting PHA production were identified: temperature, inoculum size, KH2PO4, and MgSO4.
• Optimized conditions yielded 0.647 g/L PHA, a 13.29-fold increase from 0.34 g/L, closely matching the predicted 0.630 g/L.

Conclusions:

• Bacillus paranthracis RSKS-3 is a promising candidate for cost-effective and sustainable PHA production.
• Optimization strategies significantly enhanced PHA yields, demonstrating strain's industrial potential.
• This study advances the development of biodegradable polymers from waste resources.