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Related Experiment Video
Updated: Jun 15, 2025

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
Published on: December 2, 2017
Pilot study on optimizing pressure for standardized capillary refill time measurement.
Zi-Yu Ma1, Shen Sun1, Shui-Cai Wu1
1Faculty of Environment and Life, Beijing University of Technology, China, No. 100 Pingleyuan, Chaoyang District, Beijing, 100124, China.
Optimizing pressure is key for accurate Capillary Refill Time (CRT) measurements in sepsis. Applying 10.0N-10.5N force ensures the most reliable and consistent CRT readings.
Area of Science:
- Biomedical Engineering
- Physiology
- Critical Care Medicine
Background:
- Capillary Refill Time (CRT) measurement is increasingly vital for assessing conditions like sepsis and septic shock.
- Standardizing CRT measurement requires understanding the influence of applied pressure.
- Previous methods lacked standardized pressure application, potentially affecting accuracy.
Purpose of the Study:
- To determine the optimal pressing strength for standardized Capillary Refill Time (CRT) measurement.
- To analyze the impact of varying pressure levels on CRT readings.
- To establish a reliable protocol for CRT assessment in clinical settings.
Main Methods:
- Seventeen healthy volunteers participated in CRT tests on fingertips.
- Applied forces ranged from 0.5N to 10.5N, increasing incrementally by 0.5N.
- Photoplethysmography (PPG) captured light intensity fluctuations; a specialized algorithm derived CRT.
Main Results:
- Significant differences in CRT were observed across 21 pressure levels (p < 0.0001).
- CRT measurements between 4.5N and 10.5N showed moderate consistency (ICC = 0.499).
- Pressures of 10.0N and 10.5N demonstrated strong agreement and reliability (ICC = 0.790).
Conclusions:
- A pressure range of 4.5N-10.5N is recommended for stable CRT measurements.
- Optimal consistency and reliability for CRT assessment are achieved with 10.0N-10.5N applied force.
- Findings support the development of standardized protocols for pressure-controlled CRT measurement.

