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PRNP is a pan-cancer prognostic and immunity-related to EMT in colorectal cancer

  • 0Department of Gastroenterology, The First People's Hospital of Taicang City, Taicang Affiliated Hospital of Soochow University, Taicang, Jiangsu, China.
Frontiers in Cell and Developmental Biology +

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August 22, 2024

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August 22, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

The prion protein gene (PRNP) is highly expressed in most cancers, correlating with patient prognosis and immune cell infiltration. PRNP promotes cancer cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), particularly in colorectal cancer (CRC).

Area Of Science

  • Oncology
  • Immunology
  • Genetics

Background

  • The prion protein gene (PRNP) is widely expressed across various tissues.
  • While PRNP's role in some cancers is known, a pan-cancer analysis linking it to tumorigenesis and immunity is lacking.

Purpose Of The Study

  • To conduct a pan-cancer analysis of PRNP expression and its prognostic implications.
  • To investigate the correlation between PRNP expression, tumor immunity, and immunotherapy response.
  • To explore PRNP's role in colorectal cancer (CRC) cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).

Main Methods

  • Analysis of The Cancer Genome Atlas (TCGA) Pan-Cancer dataset.
  • Immune infiltration and enrichment analyses.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
  • In vitro experiments (CCK-8, Wound healing, Transwell assays) and Western blot in CRC cells.

Main Results

  • PRNP is highly expressed in most cancers, correlating with patient prognosis.
  • PRNP expression is significantly associated with immune infiltrating cells, immunosuppressive cells, and immune checkpoints.
  • PRNP expression correlates with tumor mutation burden (TMB) and microsatellite instability (MSI) in several cancers.
  • In vitro studies show PRNP enhances CRC cell proliferation, migration, invasion, and EMT.

Conclusions

  • PRNP functions as an immune-related prognostic marker for cancer patients.
  • PRNP holds potential for predicting CRC immunotherapy outcomes.
  • PRNP promotes cancer cell proliferation, migration, invasion, and EMT, particularly in CRC.

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