Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

2.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
2.9K
Bone Remodeling01:40

Bone Remodeling

38.2K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.2K
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

3.8K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.8K
Lysosomes01:31

Lysosomes

17.0K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
17.0K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

6.2K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.2K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

3.4K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SMSC-EVs restore chondrocyte function through S1PR1-mTORC2-mediated mitochondrial fusion and GPS2-HDAC1-driven epigenetic regulation.

Osteoarthritis and cartilage·2026
Same author

Expert Consensus on Vascular Assessment and Treatment Strategies for Adult Femoral Neck Fractures.

Annals of plastic surgery·2026
Same author

Bowel wall thickness measured by intestinal ultrasound as a marker of endoscopic disease activity in patients with Crohn's disease.

Gastroenterologia y hepatologia·2026
Same author

Dynamic protein-polysaccharide hydrogels with spatiotemporal controlled delivery for brain microenvironment remodeling and neural regeneration of intracerebral hemorrhage stroke.

Biomaterials·2026
Same author

Nanoplastics exposure accelerates the progression of osteoarthritis via lysosomal destabilization-mediated pyroptosis.

Journal of advanced research·2026
Same author

Crebanine protects against ovariectomy-induced bone loss by targeting Sirt1 to interfere with NF-κB acetylation and ROS activity.

Journal of pharmaceutical analysis·2026

Related Experiment Video

Updated: Jun 15, 2025

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.0K

Lysosomal biogenesis and function in osteoclasts: a comprehensive review.

Junchen Jiang1,2, Rufeng Ren1,2, Weiyuan Fang1,2

  • 1Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Frontiers in Cell and Developmental Biology
|August 22, 2024
PubMed
Summary

Lysosomes are key cellular regulators. This review explores their function in osteoclasts (OCs) and potential for treating bone diseases.

Keywords:
autophagybone metabolic disordersbone resorptionlysosomeosteoclast

More Related Videos

Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

24.1K
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

13.1K

Related Experiment Videos

Last Updated: Jun 15, 2025

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.0K
Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

24.1K
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

13.1K

Area of Science:

  • Cell Biology
  • Bone Biology
  • Pathophysiology

Background:

  • Lysosomes are vital cellular organelles involved in degradation and signaling.
  • Lysosome dysfunction is linked to various diseases.
  • Osteoclasts (OCs) are critical for bone homeostasis, but their lysosomal roles are unclear.

Purpose of the Study:

  • To review lysosomal biogenesis mechanisms.
  • To discuss lysosome functions in osteoclast differentiation, bone resorption, and autophagy.
  • To explore therapeutic potential of targeting lysosomes for bone metabolic disorders.

Main Methods:

  • Literature review of lysosomal biology and osteoclast function.
  • Analysis of current research on lysosome-osteoclast interactions.
  • Synthesis of findings on therapeutic implications.

Main Results:

  • Lysosomes play multifaceted roles in osteoclast biology.
  • Dysregulated lysosomal activity in OCs contributes to bone disorders.
  • Targeting lysosomal pathways presents a promising therapeutic avenue.

Conclusions:

  • Understanding lysosome regulation in OCs is crucial for bone health.
  • Intervening in lysosomal function offers novel therapeutic strategies for bone diseases.
  • Further research is needed to fully exploit lysosomal biology in treating bone metabolic disorders.