Related Experiment Video
Updated: Aug 8, 2026

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
Published on: January 20, 2016
In vitro microsome- and cytosol-mediated binding of 1,2-dichloroethane and 1,2-dibromoethane with DNA
A Colacci1, M Mazzullo, G Arfellini
1Istituto di Cancerologia, Università di Bologna, Italy.
Abstract:
Metabolic activation of 1,2-dichloroethane (DCE) and 1,2-dibromoethane (DBE) to forms able to bind covalently with DNA occurs in vitro either by way of microsomal or cytosolic pathways. The involvement of these two pathways is variable with respect to species or compound tested. Rat enzymes are generally more efficient than mouse enzymes in bioactivating haloalkanes and DBE is more reactive than DCE. This parallels both the previous report on in vivo comparative interaction and the higher genotoxicity of DBE.
Related Concept Videos
Cooperative Binding of Transcription Regulators
Halogenation of Alkenes
Consider the bromination of cyclopentene. Molecular bromine is polarized in the proximity of the π electrons of cyclopentene. An electrophilic bromine atom adds across the double bond, forming a cyclic bromonium ion intermediate.
Formation of Halohydrin from Alkenes
Carboxylic Acids to Methylesters: Alkylation using Diazomethane
Electrophilic 1,2- and 1,4-Addition of HX to 1,3-Butadiene
Electrophilic 1,2- and 1,4-Addition of X2 to 1,3-Butadiene

