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Related Experiment Video

Updated: Jun 15, 2025

Author Spotlight: Aiding Research in Kidney Biology by Labeling Glomeruli in Cleared Tissues
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Ultrastructural analysis of whole glomeruli using array tomography.

Takayuki Miyaki1, Nozomi Homma2, Yuto Kawasaki1

  • 1Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan.

Journal of Cell Science
|August 22, 2024
PubMed
Summary
This summary is machine-generated.

Array tomography (AT) offers a novel method for examining the entire renal glomerulus ultrastructure. This technique overcomes limitations of traditional microscopy, aiding in the evaluation of glomerular structure and disease.

Keywords:
Glomerular pathologyPodocyteScanning electron microscopySerial sectionsThree-dimensional reconstruction

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Area of Science:

  • Nephrology
  • Microscopy
  • Pathology

Background:

  • The renal glomerulus filters blood plasma to produce primary urine.
  • Glomerular ultrastructure is crucial for filtration and disease development.
  • Transmission electron microscopy has limited observational volume, hindering analysis of specific structures and localized lesions.

Purpose of the Study:

  • To present an optimized array tomography (AT) workflow for observing complete renal glomerulus serial sections.
  • To demonstrate the utility of this AT workflow through analytical examples.
  • To provide a powerful tool for structural and pathological evaluation of glomeruli.

Main Methods:

  • Development of an array tomography (AT) workflow optimized for whole glomerulus imaging.
  • Utilizing scanning electron microscopy on serial tissue sections.
  • Analysis of complete glomerular serial sections.

Main Results:

  • The optimized AT workflow enables comprehensive observation of the entire glomerulus.
  • Analytical examples showcase the workflow's effectiveness in structural and pathological assessments.
  • The technique facilitates the study of structures like the vascular pole and low-density lesions.

Conclusions:

  • The presented AT workflow is a powerful tool for detailed glomerular structural and pathological evaluation.
  • This method is expected to yield new insights into glomerular ultrastructure and cellular components.
  • Array tomography enhances the study of kidney diseases at a ultrastructural level.