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Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

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Published on: December 6, 2012

Cell-Material Interactions in Covalent Adaptable Thioester Hydrogels.

Shivani Desai¹, Benjamin Carberry², Kristi S Anseth²

¹Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States.

ACS Biomaterials Science & Engineering

|August 22, 2024

Summary

This summary is machine-generated.

Covalent adaptable networks (CANs) mimic the extracellular matrix for cell delivery. Cytoskeletal tension from human mesenchymal stem cells drives scaffold degradation, crucial for tissue regeneration.

Keywords:

cell-mediated degradation injectable scaffolds multiple particle tracking microrheology thioester networks

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Published on: December 6, 2012

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

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Published on: September 15, 2017

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

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Published on: August 4, 2017

Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Background:

Covalent adaptable networks (CANs) are dynamic polymeric materials with stimuli-responsive cross-links.
CANs can mimic the viscoelastic properties of the native extracellular matrix (ECM).
This biomimicry is essential for promoting cell migration, proliferation, and delivery in regenerative medicine.

Purpose of the Study:

To characterize thioester-based CANs for their efficacy as injectable cell delivery vehicles.
To investigate the role of cytoskeletal tension in cell-mediated degradation of these networks.
To inform the rational design of CANs for enhanced tissue regeneration and cell delivery applications.

Main Methods:

Bulk rheology was employed to assess the mechanical properties and strain recovery of thioester CANs.
Multiple particle tracking microrheology (MPT) was used to measure cell-mediated remodeling and degradation around encapsulated cells.
Inhibiting cytoskeletal tension with blebbistatin allowed for the isolation of enzymatic degradation pathways.

Main Results:

Thioester CANs demonstrated robust rheological property recovery after high strain, mimicking injectable material properties.
Encapsulated human mesenchymal stem cells (hMSCs) degraded the CANs within approximately 4 days.
Inhibition of cytoskeletal tension significantly reduced pericellular degradation and altered cell morphology and motility.

Conclusions:

Cytoskeletal tension is a primary driver of hMSC-mediated degradation in thioester CANs.
Cellular mechanical forces play a critical role in the remodeling and breakdown of these adaptable networks.
Understanding these cell-matrix interactions is key to optimizing CANs for successful cell delivery and tissue regeneration.