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Updated: Jun 15, 2025

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
A new raspberry ketone synthesis gene RinPKS4 identified in Rubus idaeus L. by transcriptome analysis
Zhimin He1, Xihuan Yan1, Junxin Zhang1
1College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, China.
Abstract:
Raspberry ketone accounts for the characteristic aroma of the raspberry fruit. In order to explore the genes involved in raspberry ketone synthesis, the transcriptome in fruit tissues of two red raspberry varieties "Polka" and "Orange legend", were sequenced and 24213 single genes were obtained. As the red raspberry fruit ripening, genes involved in flavonoid and anthocyanin synthesis were up-regulated, while those associated with lignin synthesis were down-regulated. A gene (RinPKS4) highly related to raspberry ketone synthesis was identified by transcriptome analysis, and RinPKS4 gene was over-expressed in raspberry in order to further understand the function of RinPKS4 gene in raspberry ketone synthesis. The results showed that the gene expression level of RinPKS4 in the leaf tissues of a transgenic lines increased by about 4-fold and the content of raspberry ketone increased by 42.64% compared with the wide type. This study lays a theoretical foundation for further study on the synthesis and regulation of raspberry ketone in red raspberry.
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