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Updated: Jun 15, 2025

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Published on: March 7, 2019

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Regulation of NLRPs by reactive oxygen species: A story of crosstalk

Bjoern K Ziehr1, Justin A MacDonald1

  • 1Department of Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, Alberta T2N 4Z6, Canada.

Biochimica Et Biophysica Acta. Molecular Cell Research
|August 22, 2024
PubMed

Insights

Reactive oxygen species (ROS) are crucial for inflammasome activation, a key immune response. Understanding ROS

Area of Science:

  • Immunology
  • Molecular Biology
  • Cellular Signaling

Background:

  • Nucleotide oligomerization domain (NOD)-like receptors (NLRs) are cytosolic pattern-recognition receptors vital for host defense.
  • Inflammasomes, formed after ligand recognition by NLRs, are signaling complexes that produce pro-inflammatory cytokines.
  • Dysregulation of inflammasome signaling is linked to autoimmune, cardiovascular, metabolic, and neurodegenerative diseases.

Purpose of the Study:

  • To highlight the integral role of reactive oxygen species (ROS) in inflammasome signaling initiation and propagation.
  • To emphasize the positive feedback loop between ROS production and inflammasome activation.
  • To identify the knowledge gap concerning ROS molecular targets and effects in inflammasome activation.

Main Methods:

  • Literature review of studies on NLRP family members and inflammasome activation.
  • Analysis of the role of reactive oxygen species (ROS) in inflammasome signaling pathways.
  • Synthesis of findings across various cell types and activating stimuli.

Main Results:

  • Reactive oxygen species (ROS) are integral to the initiation and propagation of inflammasome signaling across various NLRPs.
  • ROS production and inflammasome activation form a positive feedback loop, amplifying the immune response.
  • While NLRP3 is extensively studied, evidence suggests ROS involvement extends to other NLRP family members.

Conclusions:

  • Reactive oxygen species (ROS) play a critical, conserved role in inflammasome activation.
  • A significant knowledge gap exists regarding the specific molecular targets and effects of ROS in this process.
  • Future research defining ROS-mediated NLRP activation mechanisms could yield novel therapeutic strategies for inflammasome-related diseases.
Keywords:
Cysteine oxidationInflammasomeInnate immune receptorNLRPROSRedox

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